Re: [Histonet] Section Longevity

From:Laurie Reilly

Dear All,
As Gayle suggested, many stains, eg. Perl's Prussian Blue, H & E, are not 
affected by long term storage of paraffin sections.

Some that have given unsatisfactory results are controls for 
micro-organisms. I have had old sections of controls for Gram positive and 
negative bacteria, and controls for leptospira that would not stain properly.
Now I cut fresh controls and only keep them for a few days.

           Regards,   Laurie.

At 09:06 AM 01/05/05 -0700, Gayle Callis wrote:
>Depending on the kind of staining wanted, I have stained paraffin sections 
>on slides that were stored for years and didn't have any adverse 
>effects.  I was not doing immunostaining, just special stains.
>Caveat:  some special stains may be affected by long term storage, one 
>reported is Masson's Trichchrome for collagen.  I would also be careful 
>about longer term storage for immunohistochemical stains.  Days are 
>probably not a problem.
>This has been discussed before on Histonet, check the archives for further 
>  and At 02:57 PM 1/3/2005, you wrote:
>>Hello everybody,
>>I was wondering how long paraplast sections that have been
>>mounted on slides could be left alone after drying before
>>staining.  Is there any reason they couldn't be left for days
>>or weeks?
>>Thanks for your advice,
>>Histonet mailing list
>Gayle Callis
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)
>Histonet mailing list

Mr.Laurie Reilly                                              Ph 07 4781 4468
School of Veterinary & Biomedical Science      Fax  07 4779  1526
James Cook University
Townsville  Qld. 


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