Re: [Histonet] RE: murine eosinophil immunostaining
Hi Chris and Gayle,
BMK-13 certainly works well in both human and rat, I used it for those
purposes about 10 years ago.
It's definitely worth a try on mouse.
----- Original Message -----
From: "C.M. van der Loos"
Sent: Friday, January 07, 2005 2:51 AM
Subject: [Histonet] RE: murine eosinophil immunostaining
> Hi Gayle,
> I have an antibody here against human eosinophils that stains like
> hell. The datasheet said nothing if other species are stained or not.
> Considering the huge staining intensity it seems worthwhile to test
> (with a mouse-on-mouse detection system): anti-Eosinophil Major Basic
> Protein, mouse monoclonal antibody clone BMK-13 (MonoSan 6008, see:
> www.monosan.com). The antibody stains cryostat sections, smears and
> FFPE's (after pepsin pretreatment).
> Chris van der Loos, PhD
> Dept. of Pathology
> Academical Medical Center M2-230
> Meibergdreef 9
> NL-1105 AZ Amsterdam
> The Netherlands
> phone: +31 20 5665631
> fax: +31 20 6960389
> e-mail: email@example.com
> ----- Original Message -----
> >From Gayle Callis
> Date Thu, 06 Jan 2005 09:56:29 -0700
> To Histonet@lists.utsouthwestern.edu
> Subject [Histonet] murine eosinophil immunostaining
> We are embarking on murine eosinophil staining on FFPE fixed lung (not
> short fixation time!). I will be doing other searches for available
> antibodies to detect eosinophils, but would like any recommendations
> laboratories successfully doing murine eosinophil IHC.
> 1. Whether this will work on FFPE tissue, if so, retrieval?
> 2. Are frozen sections a preference - something I would prefer to do
> 3. Antibody source
> Any other suggestions will be welcome as this is NOT a project that
> originated in our laboratory so we have no control on fixation time in
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
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