Re: [Histonet] Question about IHC Stains on Mohs frozen sections


Dear Mickie,
There is an article by Dr. John Zitelli about IHC on frozen mohs sections 
in Dermatology Surgery, 2004, 30:403-408. Hope it would be of help.
Best wishes!

At 06:21 PM 1/16/2005 +0000, you wrote:

>I would like to solicit responses from anyone currently performing 
>immunohistochemical(IHC) stains on Mohs frozen sections on a routine or 
>semi-routine basis. I am interested in information about antibodies used 
>for melanoma as well as for basal cell or squamous cell carcinomas.
>This is a relatively new area of use for IHC stains and there is growing 
>interest in the application of these technics for routine use in the 
>clinical Mohs lab.
>Any of you who know me know I have many years of histology experience 
>including performing IHC on paraffin sections, both manually and by 
>automated IHC stainers.  I am interested in use of specific antibodies, 
>including manufacturers and protocols for manual staining.
>Thank you in advance for taking the time to help me out.
>Best Regards,
>Mickie Johnson
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