RE: [Histonet] endothelial cell marker other than PECAM
I use DAKO F8 on rat ffpe tissue using proteinase k digestion for 5 min.
with labelled polymer detection.
Patsy
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret
Horne
Sent: Thursday, January 13, 2005 3:10 AM
To: wen eng
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] endothelial cell marker other than PECAM
Hi ,
I haven't started yet , but am about to try von Willebrand's factor (
Factor VIII ). I'll be doing it on rat, first in FFPE to see if it
works,
and then on osmium fixed Epon embedded. Wish me luck!
I chose Factor 8 because of a paper by Ulger et al , Anat
Histol Embryol. 2002 Feb:31(1):31-5 where he compares various
rat endothelial cell markers : Factor 8 , RECA-1, PECAM-1 , ICAM-
1, OX-43 and ZO-1.
I am buying my anti body from Biocare Medical and their tech support
suggests trying a pepsin treatment as they use it on the FFPE tissue at 37 C
for 5 min.
Hope this helps , don't hesitate to email me if you have any
more questions,
Margaret
Margaret Horne ,
Histology Teaching Assistant,
Dept. of B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave., Charlottetown,
P.E.I., C1A 4P3
Canada
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