RE: [Histonet] Xylene Substitutes


We have used Histosolve from Thermo Electron (formerly Thermo Shandon) for
over 10 years for all of our processing and staining.  We have had no
troubles with any applications including our immunos.
The only thing we use real xylene for anymore is the purge cycles on our


-----Original Message-----
From: Randolph-Habecker, Julie [] 
Sent: Thursday, January 13, 2005 3:45 PM
To: ''
Subject: [Histonet] Xylene Substitutes


My lab is considering switching to a xylene substitute for tissue processing
and other routine use. Someone had mentioned that they tried to switch to a
xylene substitute a number of years ago and they had trouble getting a few
of their antibodies to work. Has anyone else had a similar problem? If so,
what substitute did you try and what antibodies were a problem?


Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345 

 This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying, distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>