RE: [Histonet] Section Longevity
My experience has been that most dye staining methods are unaffected by the
age of the sections. Figures 8 and 9 in Biotechnic and Histochemistry
75:124-131 show fresh staining of paraffin sections cut 30 years ago.
Allen A. Smith, Ph.D.
Professor of Anatomy
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida
[mailto:firstname.lastname@example.org] On Behalf Of Laurie
Sent: Wednesday, January 05, 2005 6:54 PM
To: Gayle Callis; email@example.com; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Section Longevity
As Gayle suggested, many stains, eg. Perl's Prussian Blue, H & E, are not=20
affected by long term storage of paraffin sections.
Some that have given unsatisfactory results are controls for
micro-organisms. I have had old sections of controls for Gram positive and
negative bacteria, and controls for leptospira that would not stain
Now I cut fresh controls and only keep them for a few days.
At 09:06 AM 01/05/05 -0700, Gayle Callis wrote:
>Depending on the kind of staining wanted, I have stained paraffin sections
>on slides that were stored for years and didn't have any adverse
>effects. I was not doing immunostaining, just special stains.
>Caveat: some special stains may be affected by long term storage, one
>reported is Masson's Trichchrome for collagen. I would also be careful
>about longer term storage for immunohistochemical stains. Days are
>probably not a problem.
>This has been discussed before on Histonet, check the archives for further
> and At 02:57 PM 1/3/2005, you wrote:
>>I was wondering how long paraplast sections that have been
>>mounted on slides could be left alone after drying before
>>staining. Is there any reason they couldn't be left for days
>>Thanks for your advice,
>>Histonet mailing list
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
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Mr.Laurie Reilly Ph 07 4781
School of Veterinary & Biomedical Science Fax 07 4779 1526
James Cook University
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