[Histonet] RE: CD68 in monkey frozen tissue
A HAPPY AND HEALTHY NEW YEAR TO EVERYONE TOO!
CD68, clone PG-M1 does stain something on frozen sections. However, if
this staining result reflects the full spectrum of macrophages is a bit
doubtful. For frozens you better use CD68 clone EBM11.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
NL-1105 AZ Amsterdam
phone: +31 20 5665631
fax: +31 20 6960389
----- Original Message -----
Date Mon, 3 Jan 2005 11:37:43 +0100
Subject [Histonet] CD68 in monkey frozen tissue
HAPPY NEW YEAR TO EVERYONE !
I use the anti human CD68, KP1 from Dako on FFPE monkey tissue with
results. The same antibody is not recommended for frozens.
I tried it anyway and it is indeed difficult to get an enough strong
signal without background.
What about the other clone PG-M1 ? Is this working on frozen sections ?
Any input is very much welcome !
Novartis Pharma AG
BU Transplantation Research
Tel. +41 61 324 6730
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