[Histonet] Question about IHC Stains on Mohs frozen sections



I would like to solicit responses from anyone currently performing immunohistochemical(IHC) stains on Mohs frozen sections on a routine or semi-routine basis. I am interested in information about antibodies used for melanoma as well as for basal cell or squamous cell carcinomas.

This is a relatively new area of use for IHC stains and there is growing interest in the application of these technics for routine use in the clinical Mohs lab.

Any of you who know me know I have many years of histology experience including performing IHC on paraffin sections, both manually and by automated IHC stainers.  I am interested in use of specific antibodies, including manufacturers and protocols for manual staining.

Thank you in advance for taking the time to help me out.

Best Regards,

Mickie Johnson
Mohs Histology Temporary Services
Providing Training, Consultation and Vacation Relief to the Mohs Micrographic Surgery community and vacation relief coverage for routine histology.
web-site: www.mohshistotemp.com

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