Dear All

I am just starting a research project and need to fix mouse lungs for embedding in parafin so that I can use them for histology and immunohistochemistry. Could you please tell me the best way to fix the lungs? So far I have tried removing the lungs and then instilling 10% neutral buffered formalin into the trachea with a syringe. This seems to work but I'm not sure how reproducible the alveolar architecture will be. Also do people exsanguinate the mouse before fixing the tissues? (we've been using cervical dislocation and then just removing the lungs)
Many thanks

Andrew MacDuff
Clinical Research Fellow
Wilkie Laboratory
Medical School
Edinburgh University
Teviot Place
Edinburgh
andrew.macduff@ed.ac.uk
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