[Histonet] Humanized mouse monoclonals
Dear Histonetters,
I was wondering if anyone could provide me with a basic protocol using a
preformed complex of a humanized mouse monoclonal (human Fc ) with a
biotinylated anti-human IgG to detect antigens in human cryostat sections
- essentially, human on human IHC staining? I have several antibodies
with different total protein concentrations.
Thanks
Tom Crowell
BiogenIDEC
Cambridge, MA
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