Re: [Histonet] embarrassing question
HI Sharon:
In my experience the damage, if any, will depend on the tissue (some
are more sensitive than others) and how good a look you get at it.
Morphology that may be fine for the light microscope may not be fine for
the EM.
Cheer up, everyone I know has done this at least once!
Geoff
Sharon Cooperman wrote:
> We recently had someone perfuse mice with 0.2M Sorensen's PBS instead
> of 0.1M (with 4% formalin). I would expect the cells to shrivel, but
> when I looked at H&E's of the sections they didn't look any worse than
> some of our other blocks which were done correctly. Does anyone know
> what happens when you perfuse tissues with 2x Sorensen's PBS or have
> experience with this problem to tell me what problems I should look
> out for - eg. damage to the tissues that might not be obvious on a
> quick scan of an H&E?
>
> Thanks,
> Sharon
--
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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