RE: [Histonet] carryover on slides

From:Gary Gill

Regardless, how do you measure it?  In terms of: fouling of rinses and
frequency of replacement, cellular cross-contamination and false positives?
Acceptable compared to what?  And what's the motivation behind the question?

Gary Gill

-----Original Message-----
From: Gayle Callis [mailto:gcallis@montana.edu] 
Sent: Tuesday, January 20, 2004 9:45 AM
To: Vickroy, Jim; Histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] carryover on slides


Could you define carryover?  Do you mean cells, tissue fragments, stains
from one staining dish to another?  

At 07:40 AM 1/20/2004 -0600, you wrote:
>Has anyone ever seen any articles on what is an acceptable rate of 
>carryover on routine slides?  We have been monitoring our carryover 
>rate and our pathologists wondered if anyone had ever seen a study on 
>carryover rates.
>
>
>James R. Vickroy BS, HT (ASCP)
>Technical Supervisor, Surgical Pathology
>788-4046
>vickroy.jim@mhsil.com
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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