cut GMA blocks off any block holders and infiltrate them in fresh activated
monomer (A+C if using the JB4 kit) as if they are unembedded tissue as you
got it in the first place.  The GMA will penetrate through the polymerized
block.  Reembedd the reinfiltrated old block of tissue as for the first
embedding.
Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Barbara
Harris
Sent: Wednesday, January 21, 2004 10:40 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Recutting Old GMA tissue blocks


Need helpful tips,protocols,and experienced advise, on how to resoften
or re-embed 12 year old GMA (Glycol Methacrylate)blocked tissues.I will
be sectioning thin section for teaching purposes.Thanks in advance for
any and all responses.

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