We are experiencing the same thing.  We originally tried the antibody from
Biomedica (distributed by Medicorp in Montreal), with no success, and now
are about to try IF.  However, a colleague in Toronto is successful with the
Quidel Corporation antibody, A213, (distributed by Cedarlane) on frozen
sections, using the Ventana Benchmark, avidin/biotin system.  Despite all
claims, I haven't found anyone yet who actually has C4d working on PPFE,
just on frozen sections.  Please share your success stories!!!

-----Original Message-----
From: Featherstone, Annette [mailto:AFeatherstone@KaleidaHealth.Org]
Sent: Tuesday, January 20, 2004 1:41 PM
To: 'Instrumedics'; HistoNet Server
Subject: [Histonet] C4d antibody


Hi

Anyone out there using C4d antibody? Have to work it up and want to know if
there are any tips to make this work as they haven't been able to get it to
work right. Manufacturer recommends Alk Phos, but I was hoping to get it to
work with DAB. Any suggestions? Thanks
Annette Featherstone HT/MLT




-----Original Message-----
From: Instrumedics [mailto:info@instrumedics.com]
Sent: Tuesday, January 20, 2004 12:44
To: HistoNet Server
Subject: [Histonet] tissue carryover


One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is
that the debris generated during the trimming step is suctioned away as  it
is generated at the blockface. This substantially reduces the chances that
any sections will cling to the underside of the knife which can then can
carryover to the following case. 

Bernice
schiller@instrumedics.com



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