[Histonet] embarrassing question
We recently had someone perfuse mice with 0.2M Sorensen's PBS instead
of 0.1M (with 4% formalin). I would expect the cells to shrivel, but
when I looked at H&E's of the sections they didn't look any worse
than some of our other blocks which were done correctly. Does anyone
know what happens when you perfuse tissues with 2x Sorensen's PBS or
have experience with this problem to tell me what problems I should
look out for - eg. damage to the tissues that might not be obvious on
a quick scan of an H&E?
Thanks,
Sharon
--
Sharon Cooperman
NIH, NICHD, CBMB 301.435-7735
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892
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