In our research on acute lung injury in sheep, we have found that
partial or complete obstruction of bronchi and bronchioles is a
significant problem.  The obstructive material is composed (mainly) of
mucus, both neutral and acidic, fibrin, and cells and cell debris
including epithelial cells and neutrophils.  If possible, we would like
to use a histologic stain that will stain the mucins a distinct color
different from the cells and the fibrin, and another stain for fibrin
that will let us estimate its extent.  Even better would be a stain that
would mark mucus, fibrin, and cells differently on the same section.
Has anyone used or developed such a combination stain?  Any suggestions
on how to proceed?
 
Many thanks,
 
Hal Hawkins
UT Medical Branch, Galveston, TX, USA
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