Does anyone know of a relatively safe, commercially available substitute for
acid dichromate glassware cleaning solution? In our neuropathology lab we
prepare this solution in-house and it is used for general glassware cleaning
and especially for glassware used in silver staining methods. There are some
commercial reagents available in the US but I have yet to source one in the
UK. Any ideas?

Graham A McHardy
Pathology Dept
ARI
Aberdeen
> -----Original Message-----
> From:	histonet-request@lists.utsouthwestern.edu
> [SMTP:histonet-request@lists.utsouthwestern.edu]
> Sent:	21 January 2004 18:00
> To:	histonet@lists.utsouthwestern.edu
> Subject:	Histonet Digest, Vol 2, Issue 29 [Scanned By SOPHOS
> Anti-Virus]
> 
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> Today's Topics:
> 
>    1. Re: apotosis marker in mice (Jackie.O'Connor@abbott.com)
>    2. Re: Working Saturdays (Gareth Davis)
>    3. Hep C (Luis Chiriboga)
>    4. RE: HT Certified vs. HT non-certified (Morken, Tim - Labvision)
>    5. Recutting Old GMA tissue blocks (Barbara Harris)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Wed, 21 Jan 2004 11:25:43 -0600
> From: Jackie.O'Connor@abbott.com
> Subject: Re: [Histonet] apotosis marker in mice
> To: Susan Q Wells 
> Cc: histonet-bounces@lists.utsouthwestern.edu,
> 	histonet@pathology.swmed.edu
> Message-ID:
> 	
> 
> 	
> Content-Type: text/plain; charset="us-ascii"
> 
> I use Caspase-3 routinely for apoptosis.  I use an antibody on zinc fixed 
> paraffin embedded tissues, but there are some out there for FFPE.
> Much easier than TUNEL, and more specific.
> 
> 
> 
> 
> Susan Q Wells 
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 01/21/2004 10:17 AM
> 
>  
>         To:     histonet@pathology.swmed.edu
>         cc: 
>         Subject:        [Histonet] apotosis marker in mice
> 
> 
> Hello fellow histonetters - Does anyone have a straightforward
> protocol for an apotosis marker to use on frozen or paraffin mice
> sections.I used the TUNEL stain a number of years ago but I'm
> thinking there may be an antibody out there that works just as
> well?
> Thanks in advance for your time,
> Sue Wells HT(ASCP),QIHC
> 
> 
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> 
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Wed, 21 Jan 2004 09:28:28 -0800 (PST)
> From: Gareth Davis 
> Subject: Re: [Histonet] Working Saturdays
> To: "Weems, Joyce" ,	Histonet
> 	
> Message-ID: <20040121172828.37965.qmail@web13306.mail.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
> 
> In our lab at PathGroup, the day shift takes turns to do Saturdays.  In
> our case we only need one person, because don't do any special stains or
> IHC.  We basically just cut and get slides ready to be done early Monday
> moring.  But, the person who is scheduled to work on a Saturday gets the
> previous Monday (so that the time works).
> Good luck.
>  
> Gareth Blaeuer Davis
> Histotechinican
> PathGroup
> Nashville, Tennessee
> 
> "Weems, Joyce"  wrote:
> 
> An unofficial survey:
> 
> How many hospitals are working Saturdays? And what is the case load of 
> those
> that do?
> 
> Thanks for your help!
> 
> Joyce 
> 
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> ------------------------------
> 
> Message: 3
> Date: Wed, 21 Jan 2004 12:34:12 -0500
> From: Luis Chiriboga 
> Subject: [Histonet] Hep C
> To: Histonet 
> Message-ID: 
> Content-Type: text/plain;	charset="iso-8859-1"
> 
> Hi All
> is anyone using/trying/ have an antibody for Hep C
> Thanks
> Luis
> 
> ------------------------------
> 
> Message: 4
> Date: Wed, 21 Jan 2004 09:34:45 -0800
> From: "Morken, Tim - Labvision" 
> Subject: RE: [Histonet] HT Certified vs. HT non-certified
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> 	
> <0556BE8AC5551E4E8AF6BB9E42509BA2035762@usca0082k08.labvision.apogent.com>
> 	
> Content-Type: text/plain
> 
> Yes, the age old question. As usual, it depends......
> 
> I worked with an uncertified histotech of over 15 years experience who
> could
> do the usual special stains well, and was an excellet cutter, but who
> could
> not seem to develop methods on his own, write any meaningful procedures or
> even read very well. Basically he was one of those with one year of
> experience repeated 15 times.  Although he was not certified it was not
> for
> lack of trying - he tried five times and couldn't pass the written test
> (which goes along with the inability to develop procedures, I think, and
> he
> only attempted certification when others in the lab started a study
> group).
> But he had some skill so he was an asset to the lab as a general bench
> worker.
> 
> I have also worked with formally-trained (ie, completed a course),
> certified, very interested, but who were simply mediocre and never
> advanced.
> 
> 
> Then there are those OJT trained techs who got certified by their own hard
> work and were absolutely excellent and advanced almost at will (Indeed, I
> recently interviewed a non-certified histotech of only two years of
> experience (and no formal histotech courses) who had accomplished more in
> that two years than most techs do in their entire careers!).
> 
> So, it depends on the person. I tend to be concerned about a person who
> has
> worked in the field for 15 years and is not certified. That is due either
> to
> lack of interest, or lack of ability in some way. A person who has
> received
> the certificaiton has proven their basic skill AND at least some potential
> for personal advancement. After all, the test is a personal endeavor; the
> person has to do the lab work, the reading, and the test. You don't get
> certified just because you occupied a space in the lab for 15 years. And
> if
> the issue is trainability, probably the certified person is going to be
> more
> trainiable than someone who can't, or won't attempt to, get certified
> 
> So I guess the short answer is to look into their past very carefully and
> see what they have been doing. Never base it simply on years (long or
> short)
> or certification alone. 
> 
> Tim Morken
> 
> 
> -----Original Message-----
> From: Histolady710@aol.com [mailto:Histolady710@aol.com] 
> Sent: Tuesday, January 20, 2004 4:13 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] HT Certified vs. HT non-certified
> 
> 
> This is for histology managers !  I'm curious as to which HT you would
> hire
> - 
> a non-certified with 15 years experience or a OJT  HT (ASCP) with 2 years 
> experience.  Thanks. _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Wed, 21 Jan 2004 11:39:48 -0600
> From: "Barbara Harris" 
> Subject: [Histonet] Recutting Old GMA tissue blocks
> To: 
> Message-ID: 
> Content-Type: text/plain; charset=US-ASCII
> 
> Need helpful tips,protocols,and experienced advise, on how to resoften
> or re-embed 12 year old GMA (Glycol Methacrylate)blocked tissues.I will
> be sectioning thin section for teaching purposes.Thanks in advance for
> any and all responses.
> 
> 
> 
> ------------------------------
> 
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> 
> End of Histonet Digest, Vol 2, Issue 29
> ***************************************

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