[Histonet] RE: Block Pulling Fees
I would be interested to know if any labs, particularly in the UK, charge a
fee for pulling either blocks or slides, for example the legal profession
when they are requested in pursuit of medico-legal claims.
-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
[mailto:histonet-request@lists.utsouthwestern.edu]
Sent: 22 January 2004 15:22
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 2, Issue 31
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: How to eliminate holes in mouse brain tissue? (Gayle Callis)
2. RE: Another embedding query (Jackie.O'Connor@abbott.com)
3. RE: Another embedding query (Bauer, Karen)
4. Sakura or Leica Cassette Printer (Barbara Stancel)
5. Cole's Hematoxylin (Boneslides@aol.com)
6. Embedding technique (peptolab)
7. Embedding technique (peptolab)
8. Re: CD13 -- FFPE immunohistochemistry (Bryan Hewlett)
9. Re: Cole's Hematoxylin (Bryan Hewlett)
10. HT job opportunity, Sacramento (WWmn916@aol.com)
11. Re: Cole's Hematoxylin (Bryan Llewellyn)
12. Website help (Sonya Hogg)
13. (no subject) (Gagermeier, James)
14. RE: Website help (Nick Kirk)
15. RE: Histonet Digest, Vol 2, Issue 29 [Scanned By SOPHOS
Anti-Virus] (G.A.McHardy@arh.grampian.scot.nhs.uk)
16. Rat Micronuclei scoring (ames1@breathe.com)
17. apoptosis/necrosis IHC antibodies (lynsay jackson)
18. Re: Embedding W/WO Melted Paraffin (Gudrun Lang)
19. RE: peltier vs. refrigeration cold stage for slidingmicrotome
(Alan Bright)
20. Fw: Shur/Mount Coverslipper (Mary Parker)
21. Re: Fw: Shur/Mount Coverslipper (Joanne Mauger)
22. Re: Block Pulling Fees (Cindy Deriso)
23. RE: Pen (Stacy McLaughlin)
24. pens (BSylinda@aol.com)
25. RE: Block Pulling Fees
(Marshall Terry Dr, Consultant Histopathologist)
26. coverslipper justification (DPALLP@aol.com)
27. RE: coverslipper justification (Bartlett, Jeanine)
----------------------------------------------------------------------
Message: 1
Date: Wed, 21 Jan 2004 15:45:35 -0700
From: Gayle Callis
Subject: Re: [Histonet] How to eliminate holes in mouse brain tissue?
To: Amy Janes , Histonet@lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040121154535.00bd7798@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
AMy,
Freezing cryoprotected tissue in a cryostat will create freezing artifact
(big holes caused by water ice crystal formation with lower
temperature/slower freezing in a cryostat). You need to SNAP FREEZE.
Histonet archives is filled with suggestions on various ways to snap
freeze, do a search as this has been discussed at length recently. Sucrose
helps reduce this problem, but it can still rear its ugly head with
cryostat freezing.
Be sure you cut at a correct temperature also, cryoprotected/prefixed
tissue I find are cryosectioning well at around -26C otherwise the sucrose
oozes out like Karo syrup.
At 02:11 PM 1/21/2004 -0500, you wrote:
>Hi,
>I am writing to see if I can get some advice on how to eliminate holes I
>am seeing in my tissue.
>I am using tissue from the mouse olfactory bulb and brain for fos ICC.
>Unfortunately I have been unable to see any fos because my tissue is full
>of little holes and therefore I have no actual cells. I have determined
>that it is not the ICC that is causing the holes because they are present
>before staining. So I am assuming I am doing something wrong during
>fixation and cryoprotection. I perfuse the animal with 4% para at a rate
>of 3.5ml/min and I give the animal around 50mls. I then post fix with 4%
>para overnight and then cryoprotect with 30% sucrose until the brain drops
>(usually overnight). The brain is then imbedded in OCT and put in the
>cryostat at -20C to freeze and cut at 20microns. I typically do not
>perfuse with PBS before para but others in my lab have tried perfusing
>with and without PBS and have found no difference in tissue quality. Any
>suggestions as to how I can fix this?
>
>Thanks,
> Amy
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 2
Date: Wed, 21 Jan 2004 16:31:24 -0600
From: Jackie.O'Connor@abbott.com
Subject: RE: [Histonet] Another embedding query
To: "Stapf, Ross"
Cc: histonet@pathology.swmed.edu
Message-ID:
Content-Type: text/plain; charset="us-ascii"
As long as you have a homogenous block with all your tissue at the
appropriate plane - I don't think it much matters. If you thrust cold
tissue into molten paraffin, you're going to have tissue popping out
because of the lack of homogenous(ness?) Homogeneity? You fill in the
noun. If your mold is too cold when you put your tissue in, your block
will crumble around the edges. That's why we are artists - each block is=20
unique, and takes a skilled person with some brains (left after formalin
fixation) to determine what is best for each block. I've tried everything
- that's how I learned what NOT to do. I prefer using room temp molds
because some of the newer cassettes have a perfect leak-hole at the bottom
where the hinge was - if the mold is as hot as the paraffin, it leaks a
lot. If the mold is room temp, it chills faster, and I have less leaking=20
paraffin. Face it - it's a messy job. If we're taking a poll, I keep my=20
cassettes in the warm embedding center sans molten paraffin - but I embed=20
everything in less than an hour. I find for my purposes, the tissue is
kept at a decent temp to embed, and the paraffin it was processed in
protects it from the air. Seems to work OK for what I'm doing. If it
ain't broke, don't fix it. I'm all for heading off disaster, but I don't=20
see any looming on the horizon for us as far as embedding artifacts, etc.
Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
"Stapf, Ross"
Sent by: histonet-bounces@lists.utsouthwestern.edu
01/21/2004 03:11 PM
To: "pam plumlee" ,
cc:
Subject: RE: [Histonet] Another embedding query
At my former hospital they embedded dry with room temperature molds.
Here they embed dry with hot molds.
I think the practice of using room temperature molds at my former job
was mostly due to the main embedding tech's preference for ergonomic
comfort. They have the old Shandon Embedding Centers and she prefered
the molds to be on top of the unit, reach for the tissue with the right
hand and the mold with the left.
Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465
214-820-4110 fax
RossS@baylorhealth.edu
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam
plumlee
Sent: Wednesday, January 21, 2004 2:45 PM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Another embedding query
Since the subject of embedding styles is being
discussed...I have a new co-worker (great tech with
many years experience), that embeds with small amount
of paraffin in the holding tank and with room temp.
metal molds. I've tried it and don't like it
much...maybe I just have to get used to it. So far the
only benefit I've seen is a little less paraffin
around the blocks-hey, whats a para-trimmer for?
Anyone practice or tried this method? Thanks, Pam
__________________________________
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Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes
http://hotjobs.sweepstakes.yahoo.com/signingbonus
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 3
Date: Wed, 21 Jan 2004 17:16:47 -0600
From: "Bauer, Karen"
Subject: RE: [Histonet] Another embedding query
To: "'Jackie.O'Connor@abbott.com'" ,
"Stapf, Ross"
Cc: histonet@pathology.swmed.edu
Message-ID: <8C6E05FA69571948B461F1327CBB893E16C345@LMMAIL2>
Content-Type: text/plain; charset="iso-8859-1"
I agree with Jackie... We embed almost exactly as Jackie and we've done so
for years and years. I've been here for 13 years and we embedded the same
at the hospital I worked at before I came here. Never had any problems.
Karen Bauer HT(ASCP)
Histology Supervisor
Luther Hospital
Eau Claire, WI
-----Original Message-----
From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com]
Sent: Wednesday, January 21, 2004 4:31 PM
To: Stapf, Ross
Cc: histonet@pathology.swmed.edu
Subject: RE: [Histonet] Another embedding query
As long as you have a homogenous block with all your tissue at the
appropriate plane - I don't think it much matters. If you thrust cold
tissue into molten paraffin, you're going to have tissue popping out
because of the lack of homogenous(ness?) Homogeneity? You fill in the
noun. If your mold is too cold when you put your tissue in, your block
will crumble around the edges. That's why we are artists - each block is=20
unique, and takes a skilled person with some brains (left after formalin
fixation) to determine what is best for each block. I've tried everything
- that's how I learned what NOT to do. I prefer using room temp molds
because some of the newer cassettes have a perfect leak-hole at the bottom
where the hinge was - if the mold is as hot as the paraffin, it leaks a
lot. If the mold is room temp, it chills faster, and I have less leaking=20
paraffin. Face it - it's a messy job. If we're taking a poll, I keep my=20
cassettes in the warm embedding center sans molten paraffin - but I embed=20
everything in less than an hour. I find for my purposes, the tissue is
kept at a decent temp to embed, and the paraffin it was processed in
protects it from the air. Seems to work OK for what I'm doing. If it
ain't broke, don't fix it. I'm all for heading off disaster, but I don't=20
see any looming on the horizon for us as far as embedding artifacts, etc.
Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
"Stapf, Ross"
Sent by: histonet-bounces@lists.utsouthwestern.edu
01/21/2004 03:11 PM
To: "pam plumlee" ,
cc:
Subject: RE: [Histonet] Another embedding query
At my former hospital they embedded dry with room temperature molds.
Here they embed dry with hot molds.
I think the practice of using room temperature molds at my former job
was mostly due to the main embedding tech's preference for ergonomic
comfort. They have the old Shandon Embedding Centers and she prefered
the molds to be on top of the unit, reach for the tissue with the right
hand and the mold with the left.
Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465
214-820-4110 fax
RossS@baylorhealth.edu
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam
plumlee
Sent: Wednesday, January 21, 2004 2:45 PM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Another embedding query
Since the subject of embedding styles is being
discussed...I have a new co-worker (great tech with
many years experience), that embeds with small amount
of paraffin in the holding tank and with room temp.
metal molds. I've tried it and don't like it
much...maybe I just have to get used to it. So far the
only benefit I've seen is a little less paraffin
around the blocks-hey, whats a para-trimmer for?
Anyone practice or tried this method? Thanks, Pam
__________________________________
Do you Yahoo!?
Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes
http://hotjobs.sweepstakes.yahoo.com/signingbonus
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
********************Confidentiality Notice********************
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------------------------------
Message: 4
Date: Wed, 21 Jan 2004 23:17:45 +0000
From: "Barbara Stancel"
Subject: [Histonet] Sakura or Leica Cassette Printer
To: laurie.colbert@huntingtonhospital.com,
histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; format=flowed
Laurie, We just bought the Sakura cassette and slide autowriters. Love'em.
Love'em. Love'em.
The only difficult part was training the techs......it took us quite a while
to catch on. Me longer than the others. But once we caught on, it has been
great.
Great service from Sakura also (thanks Sharon Weyman for your hours and
hours of patience!).
Histologically yours,
Barbara
USDA, FSIS, OPHS, Eastern Laboratory, Pathology
Athens, Georgia 30604
Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie
Colbert
Sent: Wednesday, January 21, 2004 11:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Cassette Printer
Does anyone out there have or tried out the Leica IP C Cassetter Printer?
If so, can you provide any feedback? I am still trying to find a cassette
labeler that will work for us. We have tried the Shandon, the TBS, and the
new Sakura, but I wasn't totally happy with any of them.
Does anyone have info on the annual CSH meeting this year?
Laurie Colbert
Huntington Hospital
Pasadena, CA
_________________________________________________________________
There are now three new levels of MSN Hotmail Extra Storage! Learn more.=20
http://join.msn.com/?pgmarket=en-us&page=hotmail/es2&ST=1
------------------------------
Message: 5
Date: Wed, 21 Jan 2004 18:20:48 EST
From: Boneslides@aol.com
Subject: [Histonet] Cole's Hematoxylin
To: histonet@pathology.swmed.edu
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
Does anyone have a procedure for making Cole's Hematoxylin that they would
be
willing to share??
Thanks in advance!
Diane Mahovlic, HT(ASCP)
Orthopedic Pathology and Biomaterials Laboratory
The Cleveland Clinic Foundation
Cleveland, Ohio
------------------------------
Message: 6
Date: Wed, 21 Jan 2004 18:39:58 -0500
From: "peptolab"
Subject: [Histonet] Embedding technique
To: "HistoNet Server"
Message-ID: <000e01c3e078$1b744d50$95a5bd18@JEFF>
Content-Type: text/plain; charset="iso-8859-1"
I use room temperature molds for everything (Tissue Tek Plastics). The only
things that get melted on the warm plate are any needle biopsies- melt the
bottom layer, place em in and position, move to cold plate and quickly tamp
with the little tamper thingies. The larger sized rectangular molds are
banned from my lab.
I embed from the holding reservoir filled half way or less. Everything sinks
to the bottom and comes out fine, the relative chill though enables most
curettings to come out in one cake- easy to transfer to the mold.
Tricks:
TURP, I gross in a "monolayer" of prostate chips in the cassette. When
embedding, I invert the cassette over the base mold and tap, everything
falls into place (if your lucky) and there is no need to mess with the
chips further.
Gunk wrapped in lens tissue can be cooled ever so slightly, scraped off the
paper with a slide, as the layer of wax and gunk builds up on the edge, one
can warm the bottom of the slide gently and scrape it off in one piece right
into the mold.
One thing though- I sure miss my bunsen burner. It would keep us warm
during these frigid winter days.
Jeff Silverman
Southside Hospital
Bay Shore NY
------------------------------
Message: 7
Date: Wed, 21 Jan 2004 18:43:30 -0500
From: "peptolab"
Subject: [Histonet] Embedding technique
To: "HistoNet Server"
Message-ID: <001301c3e078$6376c010$95a5bd18@JEFF>
Content-Type: text/plain; charset="iso-8859-1"
I use room temperature molds for everything (Tissue Tek Plastics). They are
filled leaving 0.5-1 mm empty at the top. Saves lots of time cleaning the
blocks and everything is level and fine at the bottom.
The only things that get melted on the warm plate are any needle biopsies-
melt the
bottom layer, place em in and position, move to cold plate and quickly tamp
with the little tamper thingies. The larger sized rectangular molds are
banned from my lab.
I embed from the holding reservoir filled half way or less. Everything sinks
to the bottom and comes out fine, the relative chill though enables most
curettings to come out in one cake- easy to transfer to the mold.
Tricks:
TURP, I gross in a "monolayer" of prostate chips in the cassette. When
embedding, I invert the cassette over the base mold and tap, everything
falls into place (if your lucky) and there is no need to mess with the
chips further.
Gunk wrapped in lens tissue can be cooled ever so slightly, scraped off the
paper with a slide, as the layer of wax and gunk builds up on the edge, one
can warm the bottom of the slide gently and scrape it off in one piece right
into the mold.
One thing though- I sure miss my bunsen burner. It would keep us warm
during these frigid winter days.
Jeff Silverman
Southside Hospital
Bay Shore NY
------------------------------
Message: 8
Date: Wed, 21 Jan 2004 20:51:54 -0500
From: "Bryan Hewlett"
Subject: Re: [Histonet] CD13 -- FFPE immunohistochemistry
To: "HistoNet Server" , "Alex Knisely"
Message-ID: <000401c3e08a$50179fa0$6500a8c0@bryanmainbox>
Content-Type: text/plain; charset="iso-8859-1"
Alex,
I haven't tried it, but Novocastra list the following;
NCL-CD13-304, clone 38C12, which is reported to work on FFPE following HIER
in citrate buffer pH6.0
Worth a try!
Regards,
Bryan
----- Original Message -----
From: "Alex Knisely"
To: "HistoNet Server"
Sent: Wednesday, January 21, 2004 6:31 AM
Subject: [Histonet] CD13 -- FFPE immunohistochemistry
> CD13, also called "aminopeptidase N", is a white-cell antigen usually
> assessed by flow cytometry.
>
> I'd like to look at it in FFPE materials.
>
> A search through ABCAM has not found a reference to a commercially
> available source of an anti-CD13 antibody that works in FFPE sections.
>
> Can anyone recommend a source and protocol for such an antibody?
>
> Best thanks in advance
>
> Alex K
>
>
> Alex Knisely, MD
> Consultant Histopathologist
>
> alex.knisely@kcl.ac.uk
>
> Institute of Liver Studies
> King's College Hospital
> Denmark Hill
> London SE5 9RS UK
>
> +44 (0)20 - 7346 - 3125 telefax
> +44 (0)20 - 7346 - 4627 office
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Wed, 21 Jan 2004 21:03:28 -0500
From: "Bryan Hewlett"
Subject: Re: [Histonet] Cole's Hematoxylin
To: ,
Message-ID: <001501c3e08b$edb2f470$6500a8c0@bryanmainbox>
Content-Type: text/plain; charset="iso-8859-1"
Diane,
Go to the following for everything you need;
http://members.pgonline.com/~bryand/StainsFile/stain/hemalum/cole.htm
Regards,
Bryan
----- Original Message -----
From:
To:
Sent: Wednesday, January 21, 2004 6:20 PM
Subject: [Histonet] Cole's Hematoxylin
> Does anyone have a procedure for making Cole's Hematoxylin that they would
be
> willing to share??
>
> Thanks in advance!
> Diane Mahovlic, HT(ASCP)
> Orthopedic Pathology and Biomaterials Laboratory
> The Cleveland Clinic Foundation
> Cleveland, Ohio
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Wed, 21 Jan 2004 21:26:45 EST
From: WWmn916@aol.com
Subject: [Histonet] HT job opportunity, Sacramento
To: histonet@pathology.swmed.edu
Message-ID: <192.24f5498e.2d408ee5@aol.com>
Content-Type: text/plain; charset="US-ASCII"
We are looking for qualified histotechs (ASCP certified, but will consider
registry eligible and experienced). We are located in Sacramento, CA. We
offer great benefits and hard to beat profit sharing. Yosemite, the ocean,
Lake
Tahoe, tall redwoods, are geographical attractions in this area. We are a
busy laboratory and growing! For more information please call (916)
447-2718.
Deborah King, HT (ASCP)
Email: WWmn916@aol.com
------------------------------
Message: 11
Date: Wed, 21 Jan 2004 18:39:48 -0800
From: "Bryan Llewellyn"
Subject: Re: [Histonet] Cole's Hematoxylin
To: "Histonet" ,
Message-ID: <019601c3e091$036c1300$a370c2cf@bryand>
Content-Type: text/plain; charset="iso-8859-1"
The formula for Cole's hemalum is on StainsFile; http://stainsfile.info.
Choose staining techniques, then mordanted hematoxylin formulae.
Bryan Llewellyn
----- Original Message -----
From:
To:
Sent: Wednesday, January 21, 2004 3:20 PM
Subject: [Histonet] Cole's Hematoxylin
> Does anyone have a procedure for making Cole's Hematoxylin that they would
be
> willing to share??
>
> Thanks in advance!
> Diane Mahovlic, HT(ASCP)
> Orthopedic Pathology and Biomaterials Laboratory
> The Cleveland Clinic Foundation
> Cleveland, Ohio
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 12
Date: Thu, 22 Jan 2004 19:40:14 +1300 (NZDT)
From: Sonya Hogg
Subject: [Histonet] Website help
To: histonet@lists.utsouthwestern.edu
Message-ID: <20040122064014.13637.qmail@web14903.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I am looking for websites relating to histology
special staining techniques and am having difficulty
does anyone have any good links?? Thanks
http://personals.yahoo.com.au - Yahoo! Personals
New people, new possibilities. FREE for a limited time.
------------------------------
Message: 13
Date: Thu, 22 Jan 2004 01:56:43 -0500
From: "Gagermeier, James"
Subject: [Histonet] (no subject)
To: "'histonet@lists.utsouthwestern.edu'"
Message-ID:
<9122C182D4268F45BCB9E64A10B7331F024D1247@1upmc-msx7.isdip.upmc.edu>
Content-Type: text/plain
Basics of LCM
A few questions in regards to laser capture microdissection utilizing a
Leica AS LMD System:
In regards to buffers to place in the caps - I have routinely used RNA
later. This, however, readily crystalizes setting up issues with debris on
the microscope. First, is this an acceptable buffer, and second, what are
some other buffer alternatives.
Furthermore, when preparing slides, I generally store them in a -80 C unit.
I generally place them in a zip lock bag - not a heat sealed bag. Also, I do
not generally add a dessicant. Should:
a) I use heat-sealed bags. If not, do I need to place them in a zip lock at
all ?(i.e. can I simply put them in a slide box in the -80C unit)
b) Do I need to start using a dessicant?
I appreciate any response on these issues.
Jim Gagermeier
------------------------------
Message: 14
Date: Thu, 22 Jan 2004 07:33:38 -0000
From: "Nick Kirk"
Subject: RE: [Histonet] Website help
To: "Histonet"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Try
http://members.pgonline.com/~bryand/StainsFile/
or
http://www.nottingham.ac.uk/pathology/default.html
or
http://medlib.med.utah.edu/WebPath/HISTHTML/MANUALS/MANUALS.html
Nick Kirk
Head BMS
Histopathology
Hinchingbrooke Hospital
Huntingdon
England
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sonya
Hogg
Sent: 22 January 2004 06:40
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Website help
I am looking for websites relating to histology
special staining techniques and am having difficulty
does anyone have any good links?? Thanks
http://personals.yahoo.com.au - Yahoo! Personals
New people, new possibilities. FREE for a limited time.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 22 Jan 2004 08:46:46 -0000
From:
Subject: [Histonet] RE: Histonet Digest, Vol 2, Issue 29 [Scanned By
SOPHOS Anti-Virus]
To:
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Does anyone know of a relatively safe, commercially available substitute for
acid dichromate glassware cleaning solution? In our neuropathology lab we
prepare this solution in-house and it is used for general glassware cleaning
and especially for glassware used in silver staining methods. There are some
commercial reagents available in the US but I have yet to source one in the
UK. Any ideas?
Graham A McHardy
Pathology Dept
ARI
Aberdeen
> -----Original Message-----
> From: histonet-request@lists.utsouthwestern.edu
> [SMTP:histonet-request@lists.utsouthwestern.edu]
> Sent: 21 January 2004 18:00
> To: histonet@lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 2, Issue 29 [Scanned By SOPHOS
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> Today's Topics:
>
> 1. Re: apotosis marker in mice (Jackie.O'Connor@abbott.com)
> 2. Re: Working Saturdays (Gareth Davis)
> 3. Hep C (Luis Chiriboga)
> 4. RE: HT Certified vs. HT non-certified (Morken, Tim - Labvision)
> 5. Recutting Old GMA tissue blocks (Barbara Harris)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 21 Jan 2004 11:25:43 -0600
> From: Jackie.O'Connor@abbott.com
> Subject: Re: [Histonet] apotosis marker in mice
> To: Susan Q Wells
> Cc: histonet-bounces@lists.utsouthwestern.edu,
> histonet@pathology.swmed.edu
> Message-ID:
>
>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I use Caspase-3 routinely for apoptosis. I use an antibody on zinc fixed
> paraffin embedded tissues, but there are some out there for FFPE.
> Much easier than TUNEL, and more specific.
>
>
>
>
> Susan Q Wells
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 01/21/2004 10:17 AM
>
>
> To: histonet@pathology.swmed.edu
> cc:
> Subject: [Histonet] apotosis marker in mice
>
>
> Hello fellow histonetters - Does anyone have a straightforward
> protocol for an apotosis marker to use on frozen or paraffin mice
> sections.I used the TUNEL stain a number of years ago but I'm
> thinking there may be an antibody out there that works just as
> well?
> Thanks in advance for your time,
> Sue Wells HT(ASCP),QIHC
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 21 Jan 2004 09:28:28 -0800 (PST)
> From: Gareth Davis
> Subject: Re: [Histonet] Working Saturdays
> To: "Weems, Joyce" , Histonet
>
> Message-ID: <20040121172828.37965.qmail@web13306.mail.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> In our lab at PathGroup, the day shift takes turns to do Saturdays. In
> our case we only need one person, because don't do any special stains or
> IHC. We basically just cut and get slides ready to be done early Monday
> moring. But, the person who is scheduled to work on a Saturday gets the
> previous Monday (so that the time works).
> Good luck.
>
> Gareth Blaeuer Davis
> Histotechinican
> PathGroup
> Nashville, Tennessee
>
> "Weems, Joyce" wrote:
>
> An unofficial survey:
>
> How many hospitals are working Saturdays? And what is the case load of
> those
> that do?
>
> Thanks for your help!
>
> Joyce
>
>
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> ------------------------------
>
> Message: 3
> Date: Wed, 21 Jan 2004 12:34:12 -0500
> From: Luis Chiriboga
> Subject: [Histonet] Hep C
> To: Histonet
> Message-ID:
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi All
> is anyone using/trying/ have an antibody for Hep C
> Thanks
> Luis
>
> ------------------------------
>
> Message: 4
> Date: Wed, 21 Jan 2004 09:34:45 -0800
> From: "Morken, Tim - Labvision"
> Subject: RE: [Histonet] HT Certified vs. HT non-certified
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>
> <0556BE8AC5551E4E8AF6BB9E42509BA2035762@usca0082k08.labvision.apogent.com>
>
> Content-Type: text/plain
>
> Yes, the age old question. As usual, it depends......
>
> I worked with an uncertified histotech of over 15 years experience who
> could
> do the usual special stains well, and was an excellet cutter, but who
> could
> not seem to develop methods on his own, write any meaningful procedures or
> even read very well. Basically he was one of those with one year of
> experience repeated 15 times. Although he was not certified it was not
> for
> lack of trying - he tried five times and couldn't pass the written test
> (which goes along with the inability to develop procedures, I think, and
> he
> only attempted certification when others in the lab started a study
> group).
> But he had some skill so he was an asset to the lab as a general bench
> worker.
>
> I have also worked with formally-trained (ie, completed a course),
> certified, very interested, but who were simply mediocre and never
> advanced.
>
>
> Then there are those OJT trained techs who got certified by their own hard
> work and were absolutely excellent and advanced almost at will (Indeed, I
> recently interviewed a non-certified histotech of only two years of
> experience (and no formal histotech courses) who had accomplished more in
> that two years than most techs do in their entire careers!).
>
> So, it depends on the person. I tend to be concerned about a person who
> has
> worked in the field for 15 years and is not certified. That is due either
> to
> lack of interest, or lack of ability in some way. A person who has
> received
> the certificaiton has proven their basic skill AND at least some potential
> for personal advancement. After all, the test is a personal endeavor; the
> person has to do the lab work, the reading, and the test. You don't get
> certified just because you occupied a space in the lab for 15 years. And
> if
> the issue is trainability, probably the certified person is going to be
> more
> trainiable than someone who can't, or won't attempt to, get certified
>
> So I guess the short answer is to look into their past very carefully and
> see what they have been doing. Never base it simply on years (long or
> short)
> or certification alone.
>
> Tim Morken
>
>
> -----Original Message-----
> From: Histolady710@aol.com [mailto:Histolady710@aol.com]
> Sent: Tuesday, January 20, 2004 4:13 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] HT Certified vs. HT non-certified
>
>
> This is for histology managers ! I'm curious as to which HT you would
> hire
> -
> a non-certified with 15 years experience or a OJT HT (ASCP) with 2 years
> experience. Thanks. _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 21 Jan 2004 11:39:48 -0600
> From: "Barbara Harris"
> Subject: [Histonet] Recutting Old GMA tissue blocks
> To:
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
>
> Need helpful tips,protocols,and experienced advise, on how to resoften
> or re-embed 12 year old GMA (Glycol Methacrylate)blocked tissues.I will
> be sectioning thin section for teaching purposes.Thanks in advance for
> any and all responses.
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 2, Issue 29
> ***************************************
------------------------------
Message: 16
Date: Thu, 22 Jan 2004 09:20:03 +0000
From: ames1@breathe.com
Subject: [Histonet] Rat Micronuclei scoring
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; format=flowed; charset="utf-8"
Hi there,
I'm hoping someone can help me with a staining technique to view micronuclei
in PCEs and NCEs on rat bone marrow smears. Apparently the best technique is
the H&E stain but when I try it the haematoxylin is too strong. I have tried
reducing the concentration to 0.5% but the some of the PCEs are still
blacked out. Am I doing something wrong?
I have resolved the Eosin being overpowering by using aqueous Eosin.
I hope everyone is enjoying the New Year.
My thanks to anyone who can help,
Amy Greenhalgh
Scientist
Sequani Ltd,
Bromyard Road,
Ledbury,
HR8 1LH
------------------------------
Message: 17
Date: Thu, 22 Jan 2004 10:00:57 +0000
From: "lynsay jackson"
Subject: [Histonet] apoptosis/necrosis IHC antibodies
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; format=flowed
Hi does anyone know any companies to buy IHC necrosis/apoptosis antibodies
from?
_________________________________________________________________
Express yourself with cool emoticons - download MSN Messenger today!
http://www.msn.co.uk/messenger
------------------------------
Message: 18
Date: Thu, 22 Jan 2004 12:04:18 +0100
From: "Gudrun Lang"
Subject: Re: [Histonet] Embedding W/WO Melted Paraffin
To: "Histonetliste" ,
Message-ID: <005d01c3e0d7$7b5bf380$eeeea8c0@SERVER>
Content-Type: text/plain; charset="iso-8859-1"
In my lab we do the "dry" method for at least twenty years and have no
problems. The probes stay in the 60 degree-oven and then in the warm area of
the embedding center for about two hours, while embedding. It is cleaner to
handle.
Lang Gudrun
----- Original Message -----
From:
To:
Sent: Wednesday, January 21, 2004 4:37 AM
Subject: [Histonet] Embedding W/WO Melted Paraffin
> Hi, everyone:
> In order to settle a difference of opinion between generations of trained
> Histotechs in my lab, may I have feedback from anyone interested in
responding?
> Do you cover the blocks in melted paraffin in the embedding centre
reservior
> and hold them that way while embedding? or....
> Do you dump them in the warm reservior "dry" (not covered in melted
paraffin)
> and embed them that way?
> Do you consider this "dry" method as bad technique since a tiny biopsy
> specimen MAY not be noticed as the paraffin quickly solidifies?
> We have a dispute. I have researched every book I can find and there is
no
> reference to it anywhere. A newly trained tech that came to work for us
said
> no mention was made during her training period. Some techs did...and some
> didn't. As a tech of nearly 27 years, I find this practice to be just
asking for
> trouble. I was trained to keep everything melted. There seems to be some
> argument against keeping the cassettes in the melted paraffin, claiming it
"cooks"
> the biopsies. I don't buy it, but what are the opinions of others?
> It seems so basic to me. I hope this does not come across as frivilous=2E
> Thanks for your input.
>
> Dannie Blake HT(ASCP) Histology Lead Tech
> Fresno Community Medical Centre
> Fresno, California
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 19
Date: Thu, 22 Jan 2004 11:52:02 -0000
From: "Alan Bright"
Subject: RE: [Histonet] peltier vs. refrigeration cold stage for
slidingmicrotome
To: ,
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Dear Kathy,
We manufacture both systems the two Peltier solid state type, have
cooling outputs for a 30 X 30mm stage is 15watts @ 0șC & 40 X 40mm stage
is 25 watts @ 0șC.
The refrigerated Stage type cooling output for a 90 x 130mm stage is 300
watts @ 0șC decreasing to 125 watts @ -30șC.
With this information you will need to decide which suits the sizes and
sectioning temperature of the specimens required for sectioning best, If
you require any additional assistance, I will be only to willing to
help.
Best Regards
Alan Bright
Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com
-----Original Message-----
From: katherine-walters@uiowa.edu [mailto:katherine-walters@uiowa.edu]
Sent: 21 January 2004 14:27
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] peltier vs. refrigeration cold stage for
slidingmicrotome
Dear Histologists,
I am beginning the process of looking at cold stages for a sliding
microtome.
Does anyone have experience or opinions about the usage of peltier
stages as
opposed to refrigeration stages? I would also appreciate any
information on
which companies have these for sale.
Thank you once again,
Kathy Walters
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------------------------------
Message: 20
Date: Thu, 22 Jan 2004 06:53:50 -0500
From: "Mary Parker"
Subject: [Histonet] Fw: Shur/Mount Coverslipper
To:
Message-ID: <007c01c3e0de$67955fb0$6b01a8c0@93F1H11>
Content-Type: text/plain; charset="Windows-1252"
----- Original Message -----
From: Mary Parker
To: histonet-request@lists.utsouthwestern.edu
Sent: Thursday, January 22, 2004 6:45 AM
Subject: Shur/Mount Coverslipper
If anyone has a newer model of this automatic coverslipper (formerly Hacker,
now TBS), please tell me which mounting medium has worked best. I understand
from the sales rep that we can not use Permount on this machine. Any
comments?
Mary Parker, H.T., A.S.C.P.
Histology Manager
EPL, Inc.
P.O. Box 12766
RTP, N.C. 27709
(919) 998-9407
------------------------------
Message: 21
Date: Thu, 22 Jan 2004 07:52:35 -0500
From: "Joanne Mauger"
Subject: Re: [Histonet] Fw: Shur/Mount Coverslipper
To: mparker@epl-inc.com, histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=us-ascii
Mary,
In reference to the mounting medium for the Hacker- Permount is toluene
based. If you use xylene as a clearing agent, you should use a xylene based
mounting medium. The toluene based one will dry out real quickly, and you
will have coverslips popping off,and lots of extra work.
Jo
>>> "Mary Parker" 01/22/04 06:53AM >>>
----- Original Message -----
From: Mary Parker
To: histonet-request@lists.utsouthwestern.edu
Sent: Thursday, January 22, 2004 6:45 AM
Subject: Shur/Mount Coverslipper
If anyone has a newer model of this automatic coverslipper (formerly Hacker,
now TBS), please tell me which mounting medium has worked best. I understand
from the sales rep that we can not use Permount on this machine. Any
comments?
Mary Parker, H.T., A.S.C.P.
Histology Manager
EPL, Inc.
P.O. Box 12766
RTP, N.C. 27709
(919) 998-9407
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Thu, 22 Jan 2004 08:16:16 -0500
From: "Cindy Deriso"
Subject: Re: [Histonet] Block Pulling Fees
To: ,
Message-ID:
Content-Type: text/plain; charset=US-ASCII
And why not.. I would rather charge at the other end. Filing...
at least $5 per slide and 3 per block!
>>> "Denise Bland-Piontek" 01/21/04 11:58AM
>>>
Just wondering how many clinical labs bill for pulling blocks? Our
clinical lab just began charging $2.00 per block. Even our
pathologists
must pay when having blocks pulled for research or education. Just
wondering if this is standard? Thanks,
Denise Bland-Piontek, HTL(ASCP)
Tissue Bank & Research Administrator
WVU
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------------------------------
Message: 23
Date: Thu, 22 Jan 2004 08:26:35 -0500
From: Stacy McLaughlin
Subject: RE: [Histonet] Pen
To: 'Rebecca Barnhart' ,
histonet@lists.utsouthwestern.edu
Message-ID:
<3D502BBF5356D31184650090275B750D0346C6C8@mail.cooley-dickinson.org>
Content-Type: text/plain
We use these pens. I tried to reorder and was told that they were no longer
being manufactured. Do you mind sharing your vendor info?
Thanks,
Stacy
-----Original Message-----
From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org]
Sent: Wednesday, January 21, 2004 4:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pen
I wanted to share a wonderful discovery with everyone. We have had trouble
finding pens that stay on cassette during processing and on slide during
staining. We found a pen that works but it dulled rather quickly. We have
found a pen that works great. I got it from Ted Pella and it is the
RediSharp Plus. Hope this helps anyone that is having the same trouble as
us.
Becky
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------------------------------
Message: 24
Date: Thu, 22 Jan 2004 08:50:25 -0500
From: BSylinda@aol.com
Subject: [Histonet] pens
To: histonet@lists.utsouthwestern.edu
Message-ID: <623063F4.6295A405.00698496@aol.com>
Content-Type: text/plain; charset=iso-8859-1
We use a Statmark Pen from Statlab, that is resistant in alcohols and
xylene. Statlab Medical Products 18004423573 item # SMP-BK
Sylinda
------------------------------
Message: 25
Date: Thu, 22 Jan 2004 13:22:47 -0000
From: "Marshall Terry Dr, Consultant Histopathologist"
Subject: RE: [Histonet] Block Pulling Fees
To:
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
If techs charge for pulling blocks, do ornithologists charge for pulling
birds?
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall@rothgen.nhs.uk
------------------------------
Message: 26
Date: Thu, 22 Jan 2004 10:08:15 -0500
From: DPALLP@aol.com
Subject: [Histonet] coverslipper justification
To: histonet@pathology.swmed.edu
Message-ID: <34FA2027.1A9497BC.00042A59@aol.com>
Content-Type: text/plain; charset=iso-8859-1
Is there a general rule about the volume of slides needed to justify the
cost of an automated coverslipper? One of my pathologists seems to think
the magical number is 300 slides per day. Thanks.
Susie
------------------------------
Message: 27
Date: Thu, 22 Jan 2004 10:17:39 -0500
From: "Bartlett, Jeanine"
Subject: RE: [Histonet] coverslipper justification
To: ,
Message-ID:
Content-Type: text/plain; charset="us-ascii"
I think that whenever you can walk away from a machine while it is
performing a task and do other work, then that it is a productive and
justified item.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
DPALLP@aol.com
Sent: Thursday, January 22, 2004 10:08 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] coverslipper justification
Is there a general rule about the volume of slides needed to justify the
cost of an automated coverslipper? One of my pathologists seems to
think the magical number is 300 slides per day. Thanks.
Susie
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------------------------------
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End of Histonet Digest, Vol 2, Issue 31
***************************************
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