I am attempting to find an antibody for immunofluorescence that is the best
(from experience) in isolating endothelial cells.  This is significant for
me as I am attempting to isolate endothelail cells in order to perform laser
microdissection on these cells for further studies.  The problem I am faced
with is that an approach utilizing double staining will take too long,
allowing RNA degradation.  It is reccomended that a specific primary
biotinylated antibody be used - and then a rapid IF staining kit by Arcturus
is used.  The IF protocol appears to take 12-15 minutes.

I am trying to determine the best antibody so that I can use a single
biotinylated primary antibody with the rapid Arcturus IF protocol.  Any
reccs are appreciated. 

Thanks 

Jim Gagermeier 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet