[Histonet] Articular cartilage wrinkles
From: | "Wadsworth, Marilyn P" |
Dear Histonet,
> I am processing specimens of articular cartilage/bone from rabbit
> specimens. The tissue has been fixed and decaled using EDTA in
> formalin using the method of Kirviranta, 1985.
>
> Kiviranta, I., et al., Microspectrophotometric quantitation of
> glycosaminoglycans in articular cartilage sections stained with
> Safranin O. Histochemistry., 1985. 82(3): p. 249-55.
>
> The sections are paraffin embedded and sectioned at 5 or 6 microns.
> Upon mounting on slides, the articular cartilage appears puckered or
> wrinkled (result of swelling of cartilage when exposed to water). Is
> there anything that may be done to eliminate this artifact? Is it
> possible to float the microtomed sections on a non-aqueous solution?
> I'd appreciate any tips you may offer. Thanks
>
Maria Roemhildt, PhD
University of Vermont
Department of Orthopaedics & Rehabilitation
95 Carrigan Drive
Burlington, VT 05405
Ph. 802-656-3823
fax: 802-656-4247
maria.roemhildt@med.uvm.edu
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>