celestine blue - iron alum

From:Bryan Llewellyn

I believe the original paper introducing iron alum mordanted celestine blue
B was:

Proescher, F. and Arkush, A. S. (1928),
Metallic lakes of the oxazines (gallamin blue, gallocyanin and coelestin
blue) as nuclear stain substitutes for hematoxylin.
Stain Technology, v3, p28-38.

Coelestin is an older way of spelling celestine.  This method was revived as
an alum hematoxylin substitute during the shortage of that dye in the 1970s.
Most people who did work on hematoxylin substitutes then, referred to this
paper as the original.

It appears that Lendrum and McFarlane added glycerol to the solution
(according to Gray), and used their modification in the celestine blue
hemalum sequence.

Lendrum and McFarlane, (1940),
Journal of pathology and bacteriology, v50, p381


Bryan Llewellyn





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