Besides the details below, I should have mentioned that the primary is
incubated for an hour at room temp.

Tim

-----Original Message-----
From: Morken, Tim [mailto:tim9@cdc.gov]
Sent: Tuesday, January 21, 2003 8:34 AM
To: 'Bonnie P Whitaker'; histonet
Subject: RE: SV 40 T-Ag


Hi Bonnie, we are using this antibody from Oncogene:

SV40 T Ag (Ab-2), Oncogene cat# DP02, clone: PAb416

We use it with steam AR in BioGenex Citra buffer. (boil citra buffer in
microwave, then place slides in hot buffer and put in steam chamber for 20
min. Cool in running distilled water, then on with the run). We use it at
1:500, with DAKO LSAB2 alk phos kit.

Tim Morken EMT(MSA), HTL(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology
MS-G32 
1600 Clifton Road
Atlanta, GA 30333

PH 404-639-3964
FAX 404-639-3043
email tim9@cdc.gov 

-----Original Message-----
From: Bonnie P Whitaker [mailto:Bonnie.P.Whitaker@uth.tmc.edu]
Sent: Monday, January 20, 2003 5:51 PM
To: histonet
Subject: SV 40 T-Ag


Hi All,

Anyone using this (from Oncogene)?  If so, please share protocol info with
me.  I have VERY limited control to work with and need to hit it as close as
possible at the 1st try.

Thanks!!

Bonnie Whitaker
University of Texas Medical School at Houston
6431 Fannin Street
MSB 2.234
Houston, Texas  77030
Phone 713.500.5363
Fax 713.500.0733