Michaela,

One of the most common problems with GMA (JB-4), is the loss of sections from
the slide after immersion in alcohol's over 70%.  After any staining procedure
just bring them to 50% ETOH and let them air dry, after being air dried dip in
xylene and coverslip.

best regards.
Robert Schoonhoven


Michaela Fr#253#zkov#225# wrote:

> Dear Roger,
> I use 2 um sections. So it might be OK.
> But I use only water for sticking sections to slides (by reason of other
> method- lectins tests). And than, in H&E, I have problem with staying
> sections on slide. So I use only short times- 3 min. Do you know any other
> method of sticking? I somewhere read about sticking sections with JB-4
> solution A...
>
> Thanks you very much for any idea.
> Michaela Fryzkova