Re: Trichrome staining.

From:"J. A. Kiernan"

Tony raises an interesting point. I haven't been able
to find the original publication of iron-celestine
blue followed by haemalum, which has been popular
for many years in the English-speaking world outside
North America. There are plenty of published references
but all the ones I've seen cite secondary sources.

Even the superb 796-page fifth edition of "The 
Theory and Practice of Histological Techniques" 
(Bancroft & Gamble, 2002) gives a procedure with
no references or even speculation about how the
method works. There are plenty of old papers about
iron-celestine blue B alone as a nuclear stain. Why
is it advantageous to follow it up with a haemalum?
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Tony Henwood:
> Remember that Celestine Blue is made up in 5% iron alum (ferric ammonium sulphate). So is its stability, in the face of the trichrome
> acidic stains, due to an iron mordant rather than the celestine blue dye itself. ?very much like an Iron Haematoxylin stain like
> Weigert's!!
> I like  the Celestine blue/Haematoxylin sequence as well.
> Just a thought!!
> Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager
> The Children's Hospital at  Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
>     -----Original Message-----
>     From: Ian Montgomery []
>     Sent: Thursday, 30 January 2003 1:59
>     To:
>     Subject: Trichrome staining.

Ian Montgomery: 
>     Although a well executed Weigert is superb for a Masson I still stand by my
>     Celestine blue/Haematoxylin sequence. Over the years I've used it repeatedly and always
>     get beautiful staining. If like me your in the process of staining ~500 slides with a Masson
>     then the ease and reproducibility of the Celestine blue/Haematoxylin sequence has a lot to
>     commend it. 
>     Ian.
>     Dr. Ian Montgomery,
>     Histotechnology,
>     Graham Kerr Building,
>     Institute of Biomedical & Life Sciences,
>     University of Glasgow,

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