Re: Trichrome staining.
Tony raises an interesting point. I haven't been able
to find the original publication of iron-celestine
blue followed by haemalum, which has been popular
for many years in the English-speaking world outside
North America. There are plenty of published references
but all the ones I've seen cite secondary sources.
Even the superb 796-page fifth edition of "The
Theory and Practice of Histological Techniques"
(Bancroft & Gamble, 2002) gives a procedure with
no references or even speculation about how the
method works. There are plenty of old papers about
iron-celestine blue B alone as a nuclear stain. Why
is it advantageous to follow it up with a haemalum?
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
> Remember that Celestine Blue is made up in 5% iron alum (ferric ammonium sulphate). So is its stability, in the face of the trichrome
> acidic stains, due to an iron mordant rather than the celestine blue dye itself. ?very much like an Iron Haematoxylin stain like
> I like the Celestine blue/Haematoxylin sequence as well.
> Just a thought!!
> Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager
> The Children's Hospital at Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> -----Original Message-----
> From: Ian Montgomery [mailto:email@example.com]
> Sent: Thursday, 30 January 2003 1:59
> To: firstname.lastname@example.org
> Subject: Trichrome staining.
> Although a well executed Weigert is superb for a Masson I still stand by my
> Celestine blue/Haematoxylin sequence. Over the years I've used it repeatedly and always
> get beautiful staining. If like me your in the process of staining ~500 slides with a Masson
> then the ease and reproducibility of the Celestine blue/Haematoxylin sequence has a lot to
> commend it.
> Dr. Ian Montgomery,
> Graham Kerr Building,
> Institute of Biomedical & Life Sciences,
> University of Glasgow,
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