I do extensive CD31 staining for microvessel density on zinc fixed (Streck Tissue fixative) paraffin sections.
I use Pharmingen's CD31 primary #553370 at 1:100, with an overnight incubation at 4C.
The staining is beautiful. I will forward my procedure if you are interested.
Jacqueline M. O'Connor HT(ASCP)
Global Pharmaceutical Research and Development
Luis Chiriboga <Luis.Chiriboga@med.nyu.edu>
01/24/2003 11:26 AM
To: Histonet <email@example.com>
Subject: Quick clarification for Ulex europaeus
To all of you who responded to my ulex post, just wanted to clarify why switching to the ulex. Using mouse w/cell line model
CD31 fixation/processing not adequate, could not get labeling after several different ab's and lots of experimentation
CD34 couldn't get labeling in the small neo-vessels
Fac8 in our model, way to much background. impossible to read
SMA works great but not supported by literature as a method for MVD