Re: Plant Histology

From:Philip Oshel


"Botanical Microtechnique and Cytochemistry" G.P. Berlyn and J.P. 
Miksche. The edition I have was published by Iowa State Univ. Press, 
1975. There may be a newer edition by some other publisher.
Also, most textbooks on Plant Anatomy/Morphology or Structural Botany 
have brief sections on preparing plant parts for microscopy, and the 
standard EM texts have sections on preparing plants for EM. See also 
Steven Paddock's book "Confocal Microscopy: Methods and Protocols", 
Chap. 6 "Confocal Microscopy of Plant Cells"; Humana Press 1999. And 
"Methods in Plant Electron Microscopy and Cytochemistry", W.V. 
Dashek, Humana Press 2000. These are all I have to hand -- there are 
more in the libraries.
As for decomposing cellulose ... if you don't have to use something 
controlled, go for a hike in the woods, and pick up some moist, 
rotting wood from a fallen tree. There will be plenty of 
cellulose-digesting fungi in that.
Then of course, there are always termites ...


>Hello everyone !
>One of our customers (an agriculture engineer)  wants to take tree
>sections. They are
>investigating tree grafts like roses, & willows. They want to take 4-5
>micron sections and they don't know anything about
>processing of plant tissues.  I am not familiar with plant processing
>either and was wondering if anyone out there could enlighten me
>on the topic of plant processing, processing schedules and anything
>different one would need to have when performing embedding,
>microtomy and staining. Is there a book specifically designated to plant
>processing , microtomy & staining ? One more thing is what
>can be used to decompose cellulose?
>Your  help as always is greatly appreciated !
>Best regards,
>Mark Lewis
>Product Specialist
>Thermo Shandon
>171 Industry Drive, Pittsburgh, PA 15275 USA
>Direct: (412) 747-4013
>Fax: (412) 788-1097
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Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

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