There are at least two good books on plant
microtechnique:

1. Berlyn GP & Miksche JP 1976. Botanical Microtechnique
  and Cytochemistry. Ames: Iowa State Univ. Press.
  Hardback, 326 pages. This is excellent; obtainable
  from the publisher at a remarkably low price.
  One of the authors is Prof of Wood Anatomy at Yale.
2. Ruzin SE 1999. Plant Microtechnique and Microscopy
  New York: Oxford Univ. Press. Paperback, 322 big
  pages. Contains information on immunohistochemistry
  and nucleic acid hybridization that's not in B&M.

-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/
____________________________________________________
mark.lewis@thermo.com wrote:
> 
> Hello everyone !
> 
> One of our customers (an agriculture engineer)  wants to take tree
> sections. They are
> investigating tree grafts like roses, & willows. They want to take 4-5
> micron sections and they don't know anything about
> processing of plant tissues.  I am not familiar with plant processing
> either and was wondering if anyone out there could enlighten me
> on the topic of plant processing, processing schedules and anything
> different one would need to have when performing embedding,
> microtomy and staining. Is there a book specifically designated to plant
> processing , microtomy & staining ? One more thing is what
> can be used to decompose cellulose?
> 
> Your  help as always is greatly appreciated !
> 
> THANK YOU ALL !!
> 
> Best regards,
> 
> Mark
> 
> Mark Lewis
> Product Specialist
> Thermo Shandon
> 171 Industry Drive, Pittsburgh, PA 15275 USA
> Direct: (412) 747-4013
> Fax: (412) 788-1097
> E-mail: mark.lewis@thermoshandon.com
> 
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