Re: PAS-trichrome (Boss etc)

From:"J. A. Kiernan"

Aziz Ahmed Jafri answered:
> We tried it once, didn't look good.
> > My boss has asked me to stain heart muscle with a
> > PAS-Trichrome stain.

"Didn't look good" is exactly what to expect
from an attempt to combine PAS with a trichrome.

The person who should be asking these questions
is the "my boss" of the earlier enquiry. He/She
evidently knows nothing about either PAS or trichrome 
stains. This boss should do some reading - buy a
few books, or go and read them in a library.

A boss should not expect her/his servants to be 
better educated than h'self.  

John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

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