Thanks Cindy!

Phyllis






>From: Cindy/Rick DuBois 
>To: histonet@pathology.swmed.edu
>CC: lsmallwo@ppmh.org, HornHV@archildrens.org, Stancelb@msn.com, 
>rfail@toolkitmail.com, azdudley@hotmail.com, ZumboR@email.cs.nsw.gov.au, 
>salvegsd@webtv.net, JBurrill@criver.com, histo20@hotmail.com, 
>CBrown@agr.gov.sk.ca, csu15397@mail.claytonstate.net
>Subject: McDonald Gram Procedure
>Date: Fri, 24 Jan 2003 13:48:10 -0800 (PST)
>
>Below is the procedure for the McDonalds Gram Stain.  All credit for this 
>technique goes to American Mastertech.  It is a staining kit they prepare.  
>They can be reached via internet:  www.mastertechs.com, or phone 
>(800)860-4073.  The item number for the kit is KTMGS.
>
>1.  Flood slides for 1  minute with Gentian violet.
>2.  Rinse in water.
>3.  Flood slides with Gram's Iodine, let stand 1 minute.
>4.  Rinse.
>5.  Decolorize in Gram's decolorizer until color no longer                
>bleeds off of slide.
>6.  Stain with Carbol Fuchsin for 90 seconds (we find that 60 seconds works 
>just as well).
>7.  Rinse quickly.
>8.  Stain with Tartrazine Solution for 15 seconds, (0ur docs like lots of 
>yellow for background so we stain 30 seconds).
>9.  Dehydrate quickly, clear thru xylene or xylene substitute and 
>coverslip.
>
>RESULTS:
>  GRAM POSITIVE:  BLUE
>  GRAM NEGATIVE:  RED
>  OTHER TISSUE:   YELLOW
>   NUCLEI:        RED.
>
>Have fun with this,
>Cindy DuBois, HT
>Delta Pathology Associates
>Stockton, CA  95207
>(209)477-4432


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