You have probably thought of this, but just in case - how are you blueing
the haematoxylin? If you are in a soft-water area, tap water alone won't
work; you need to use lithium carbonate or Scott's tap-water substitute, or
some other alkaline mix.

Lesley Weston.


on 28/01/2003 4:14 AM, Demarinis,Carolyn at cdemarinis@SARATOGACARE.ORG
wrote:

> Each time we perform a Masson Trichrome stain, the nuclei are bright red.  I
> have used Polyscientific and Sigma kits, with both Weigerts and Harris
> Hematoxylin.  Can anyone offer any suggestions?
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