Luis,

Instead of using the Ulex abs which are polyclonals and elicit high background 
why not use abs to VWF or CD31 to identify vascular endothelial cells.

Philip Seifert, MS, HTL(ASCP)
Research Associate
Massachusetts Institute of Technology
Biomedical Engineering Center Histology Unit
Building 56-338
77 Massachusetts Avenue
Cambridge, MA 02139
USA



Quoting Luis Chiriboga :

> Hi All
> I dong some lectin IHC and was curious as to what people are using.  want to
> use ulex to label brain vasculature and determine micro-vessel density.
> Would you recommend the "biotinylated ulex" or an "anti-ulex antibody"  The
> literature is not so clear ( writers just don't put in a lot of effort into
> writing  IHC material & methods)
> 
> Thanks In advance
> Luis
>