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Believe it or not.....  I have a copy available that I could snail mail.

regards.
Robert Schoonhoven

MaryLou wrote:

> Techniques in Plastic for Light Microscopy
> National Society for Histotechnology - Region IV Symposium
> June 25, 1983   Ann Arbor, Michigan
> Alexandra N. Brady, M.S., MT/HTL (ASCP)
> Robert Schoonhoven, HTL (ASCP)
>
> I like the Lee's Methylene Blue -Basic Fuchsin for an H&E look-a-like.
>
> 1) Stock methylene blue
>               0.5 g methylene blue  (CI 52015)
>               400 mL distilled water
>
> 2) Stock basic fuchsin
>              0.5 g basic fuchsin    (CI 42510)
>              400 mL distilled water
>
> 3) Stock phosphate buffer 0.1 M   pH 6.8
>             49 mL  0.2 M sodium phosphate dibasic anhydrous (28.4 g
> Na2H2PO4 in 1 liter DH2O)
>             51 mL  0.2 M  sodium phosphate monobasic monohydrate
> (27.8 g NaH2PO4-H2O in 1 liter DH2O)
>             100 mL distilled water
>
> 4)  95% ethyl alcohol
>
> 5)  Working methylene blue-basic fuchsin
>             12.0 mL stock methylene blue
>             12.0 mL stock basic fuchsin
>             21.0 mL stock phosphate buffer
>             15.0 mL 95% ethyl alcohol
> DISCARD AFTER USE
>
> Procedure:
> 1)  Stain in working solution for 10 seconds
>
> 2)  Rinse in distilled water
>
> 3)  Dip in 95% alcohol quickly with agitation to remove background
> stain
>
> 4) Rinse in water
>
> 5) Dry and mount
>
> Results: Nuclei - blue
>              Cytoplasm, cilia, certain cell granules - shades of red
> and pink
>               Cartilage and most cell granules - blue to purple
>
> Reference:  Sorvall Instruction Manual, Staining Procedures for
> Plastic Embedded Tissue, DuPont Company, Instrument Products,
> Biomedical Div.,  July, 1979
>
>
>> Date: Thu, 30 Jan 2003 16:01:38 +0100
>> X-PH: V4.1@mailhub3
>> From: =?iso-8859-2?Q?Michaela_Fr=FDzkov=E1?=
>> 
>> Subject: staining JB-4 sections
>> To: histonet 
>>
>> Dear Colleagues,
>> I need an advice about staining sections embedded in JB-4 Plus. I
>> embed in
>> this medium and now I need stain my sections. I wanted to use
>> regular
>> haematoxylin-eosin staining, but it doesn#180#t work. Do you know
>> something
>> about that? Can you recommend me some other staining?
>>
>> Thank you, very much.
>> Michaela Fryzkova
>>
>> Department of Parasitology
>> Faculty of Science
>> Charles University
>> Prague
>> Czech Republic
>

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Believe it or not.....  I have a copy available that I could snail
mail.
regards.

Robert Schoonhoven
MaryLou wrote:
Techniques in Plastic for Light Microscopy

National Society for Histotechnology - Region IV Symposium

June 25, 1983   Ann Arbor, Michigan

Alexandra N. Brady, M.S., MT/HTL (ASCP)

Robert Schoonhoven, HTL (ASCP)
I like the Lee's Methylene Blue -Basic Fuchsin for an H&E look-a-like.

1) Stock methylene blue

             
0.5 g methylene blue  (CI 52015)

             
400 mL distilled water
2) Stock basic fuchsin

            
0.5 g basic fuchsin    (CI 42510)

            
400 mL distilled water
3) Stock phosphate buffer 0.1 M   pH 6.8

           
49 mL  0.2 M sodium phosphate dibasic anhydrous (28.4 g Na2H2PO4 in
1 liter DH2O)

           
51 mL  0.2 M  sodium phosphate monobasic monohydrate  (27.8
g NaH2PO4-H2O in 1 liter DH2O)

           
100 mL distilled water
4)  95% ethyl alcohol
5)  Working methylene blue-basic fuchsin

           
12.0 mL stock methylene blue

           
12.0 mL stock basic fuchsin

           
21.0 mL stock phosphate buffer

           
15.0 mL 95% ethyl alcohol

DISCARD AFTER USE
Procedure:

1)  Stain in working solution for 10 seconds
2)  Rinse in distilled water
3)  Dip in 95% alcohol quickly with agitation to remove background
stain
4) Rinse in water
5) Dry and mount
Results: Nuclei - blue

            
Cytoplasm, cilia, certain cell granules - shades of red and pink

             
Cartilage and most cell granules - blue to purple
Reference:  Sorvall Instruction Manual, Staining Procedures for
Plastic Embedded Tissue, DuPont Company, Instrument Products, Biomedical
Div.,  July, 1979

 
Date: Thu, 30 Jan 2003 16:01:38 +0100

X-PH: V4.1@mailhub3

From: =?iso-8859-2?Q?Michaela_Fr=FDzkov=E1?= <michaela.fryzkova@centrum.cz>

Subject: staining JB-4 sections

To: histonet <histonet@pathology.swmed.edu>
Dear Colleagues,

I need an advice about staining sections embedded in JB-4 Plus. I embed
in

this medium and now I need stain my sections. I wanted to use regular

haematoxylin-eosin staining, but it doesn#180#t work. Do you know something

about that? Can you recommend me some other staining?
Thank you, very much.

Michaela Fryzkova
Department of Parasitology

Faculty of Science

Charles University

Prague

Czech Republic



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