RE: agar embedding
Dear Laurie,
Please have a look at:
Kerstens et al. J. Histochem. Cytochem 48:709-718, 2000. E-mail address of
first author is in the paper. I am sure Harold Kerstens will answer your
specific questions.
Chris van der Loos
Academic Medical Center
Dept. of Cardiovascular Pathology
Amsterdam, The Netherlands
Laurie wrote:
>Date: 14 Jan 2003 18:27:11 -0600
>From: Laurie Reilly
>Subject: Agar embedding
>
>Histonetters,
>Could somebody please provide me with a protocol for embedding tiny
>specimens in agar and then processing the agar block through ethanol and
>xylene and into paraffin. My attempts so far have produced severely shrunken
>and hard lumps of unrecognisable agar.
>Thanks in advance.
>Regards, Laurie.
>
>Mr.Laurie Reilly
>Physiology & Pharmacology
>Aust.Inst.of Tropical Vet.& Animal Sc.
>James Cook University
>Townsville Qld. 4811
>Australia.
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