RE: Trichrome staining.

From:Tony Henwood

Remember that Celestine Blue is made up in 5% iron alum (ferric ammonium sulphate). So is its stability, in the face of the trichrome acidic stains, due to an iron mordant rather than the celestine blue dye itself. ?very much like an Iron Haematoxylin stain like Weigert's!!
I like  the Celestine blue/Haematoxylin sequence as well.
Just a thought!!

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Ian Montgomery []
Sent: Thursday, 30 January 2003 1:59
Subject: Trichrome staining.

        Although a well executed Weigert is superb for a Masson I still stand by my Celestine blue/Haematoxylin sequence. Over the years I've used it repeatedly and always get beautiful staining. If like me your in the process of staining ~500 slides with a Masson then the ease and reproducibility of the Celestine blue/Haematoxylin sequence has a lot to commend it.

Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883

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