RE: Trichrome staining.- celestine blue or just iron alum?

From:Tony Henwood

RE: Trichrome staining.- celestine blue or just iron alum?


Histonet is wonderfull.

I knew someone would have looked into this and all we had to do was wait for the answer and the waiting time was less than 48 hours!!

Histonet is DEFINITELY a Brilliant resource.

Thanks Brian and all others who are willing to share their experience. Keep it up.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Bryan Llewellyn []
Sent: Friday, 31 January 2003 3:55
To: Histonet
Subject: Re: Trichrome staining.

----- Original Message -----
From: "J. A. Kiernan" <>
 There are plenty of old papers about
> iron-celestine blue B alone as a nuclear stain. Why
> is it advantageous to follow it up with a haemalum?
> --

When I was a student in paleolithic times in London, it was commonly
believed that the celestine blue-hemalum sequence was simply a fast and
convenient way to get an iron hematoxylin stain.  Celestine blue is made up
in iron alum and the iron attaches to chromatin.  The hematein in Mayer's or
Cole's hemalum then replaces the celestine blue.  The immediate question, if
that is so, is why use celestine blue, why not just use iron alum?  In fact,
this is how I have applied the stain for about 30 years.  It works quite

Bryan Llewellyn

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