RE: Re-Immuno control sections

From:"Dawson, Glen"


I keep a bank of controls.  The antibodies that need fresh cut controls have
a smaller # cut ahead of time so that I use them up quicker.  If I had to
cut controls at the same time I cut each IHC that I do, I wouldn't come
close to getting everything done in my 8 hr. shift.


-----Original Message-----
From: clarke.ian []
Sent: Wednesday, January 29, 2003 11:49 AM
To: histonet
Subject: Re-Immuno control sections

Hi all,
At present we cut our control sections for immuno at the same time as the
test which can be very time consuming when you have a big run of immuno's.We
would like to have a bank of control slides so all we would need is to cut
the tests as we already do this in special stains.What do other people do
and is there some reason why this might not be a good idea?I have thought
about the loss of antigenicity as a time factor but from papers I have read
they talk about the section being stored for months.However the time factor
does not seem to be a problem for tissue blocks.
 Another item I am looking for is the e-mail address of Sherwood Medical Co
of Saint Louis if they have one.
Thanks in advance
Ian Clarke
Histopathology/Cytopathology Department
Craigavon Area Hospital
Northern Ireland

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