RE: Protocol for plauque stain on aorta and heart?

From:"Molinari, Betsy"


 I had the same request from a pathologist here. He wanted to perfuse the tissue then stain it without any further processing .  After much investigation, without much in the way of information, he has shelved the idea for the time being. He was interested in using ORO as well.

Good luck.

Betsy Molinari HT (ASCP)

Texas Heart Institute

Houston,Texas 77030



-----Original Message-----
From: []
Sent: Thursday, January 23, 2003 5:57 PM
Subject: Protocol for plauque stain on aorta and heart?



The lab I work for has been asked to perform a stain on the aorta and heart tissue (animal tissue) looking for plauques. My suppervisor believed she had done this in the past on non embeded tissues. I searched through the histonet archives, however I was unable to open the "hits" to obtain more information. I believe this may either be en face staining or ORO staning. If anyone knows of protocols for the processing and staining of these tissues we would greatly appreciate your help. Also, if there are issues with opening results from histonet archives?

Thank you,

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