RE: PAS-trichrome (Boss etc)

From:"Monson, Frederick C."

RIGHT ON, John!!!!!

Every technician or technologist should have the ASCP and/or HT? right to
respond to a request with, "Where's the protocol?".  

Every technician who invents a protocol should be the first in line as
either the primary or senior author on the first paper that utilizes it.

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone:  610-738-0437
An FEI (Quanta 400 and Technai 12), 
Oxford INCA Energy 400, and 
Olympus FV-300 Shop.

-----Original Message-----
From: J. A. Kiernan []
Sent: Friday, January 24, 2003 1:10 AM
Subject: Re: PAS-trichrome (Boss etc)

Aziz Ahmed Jafri answered:
> We tried it once, didn't look good.
> > My boss has asked me to stain heart muscle with a
> > PAS-Trichrome stain.

"Didn't look good" is exactly what to expect
from an attempt to combine PAS with a trichrome.

The person who should be asking these questions
is the "my boss" of the earlier enquiry. He/She
evidently knows nothing about either PAS or trichrome 
stains. This boss should do some reading - buy a
few books, or go and read them in a library.

A boss should not expect her/his servants to be 
better educated than h'self.  

John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

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