RE: PAS-trichrome

From:Tony Henwood

RE: PAS-trichrome

Another suggestion:

Do a PAS followed by a Picro-aniline blue.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: J. A. Kiernan []
Sent: Thursday, 23 January 2003 17:18
To: Monson, Frederick C.
Cc: ''; List-HistoPath (E-mail)
Subject: Re: PAS-trichrome

For what it's worth,
I'd just like to add my 100% support
to Fred's good advice.
John A. Kiernan
The University of Western Ontario
Fred Monson wrote:
Hi Tom,
I would first try a PAS-H&E to get an idea of what you will get.
Then show it to your boss.  If s/he still wants it with a
do the PAS first and follow with your choice.  The magenta of the
Pararosaniline will be doused by the Aniline blue of Mallory's,
maybe not by much in Patay's.  With Mallory's in heart muscle,
however, you might still get some magenta glycogen if the blue is
properly 'differentiated'.
I have done Gomori's Aldehyde Fuchsin prior to Mallory's to add
good color to elastic fibers in loose connective tissue.
Good Luck,
Frederick C. Monson, PhD
West Chester University of Pennsylvania

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