I developed a sure-fire practice when I was a young graduate student and had
to get to the bar/pub at the right time each day.

		No matter the percentage, I did the following.  e.g. for 4%
aqueous gelatin:

		1.  Heat 100ml HOH to 40-45 degrees C while stirring
constantly on a magnetic hot plate.
		2.  While the water stirs, I learned to sprinkle the
granular gelatin on the surface of the spinning fluid so that each granule
was separately wetted.  Once wetted, a granule leaves the surface and
becomes suspended in the fluid column.
		3.  Once all dry material is suspended, I continued stirring
until no particulates were visible.  This usually took no more than 30min
past the time that I finished sprinkling the dry material.  Of course, if
autoclaving was required, then I did so immediately after wetting the dry
material.  I have only dumped dry gelatin into water as a bolus once in my
life (not including Jello, which one dumps into boiling water).  Then, the
gelatin formed a clump that didn't dissolve even when I brought the
suspension to a boil.
	
	The above always works for ME, and I assume you meant "DI" water,
but my method worked with any aqueous vehicle.

Regards,

Fred Monson
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone:  610-738-0437
eMail:  fmonson@wcupa.edu
An FEI (Quanta 400 and Technai 12), 
Oxford INCA Energy 400, and 
Olympus FV-300 Shop.

	

-----Original Message-----
From: abishop@sfsu.edu [mailto:abishop@sfsu.edu]
Sent: Friday, January 17, 2003 6:31 PM
To: HistoNet@pathology.swmed.edu
Subject: Haupt's solution


Does anyone know how long it takes to dissolve gelatin in DI? Thanks

A.Bishop
SFSU