Melanie, For HIV detection we use the DAKO monoclonal antibody HIV(p24)cat#
M857 for all formalin-fixed, paraffin-embedded samples. We use it with
protienase K digestion (0.1mg/ml in 0.2M TRIS buffer pH 7.5) for 15 min at
room temp, primary incubated at 1:10 for one hour. Detection is with DAKO
LSAB2 Alk phos kit and DAKO fast red.

Tim Morken EMT(MSA), HTL(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology
MS-G32 
1600 Clifton Road
Atlanta, GA 30333

PH 404-639-3964
FAX 404-639-3043
email tim9@cdc.gov 



-----Original Message-----
From: Trivett Melanie [mailto:Melanie.Trivett@petermac.org]
Sent: Thursday, January 23, 2003 9:13 PM
To: HistoNet Server
Subject: HIV testing of paraffin blocks



Does anyone out there know if is possible to determine HIV status on a
formalin-fixed paraffin embedded sample?  Perhaps by PCR methods? I have
seen a few references but wonder if this is being done in a diagnostic
setting.

Any information you can provide would be greatly appreciated.


Many thanks,

Melanie