RE: HIV testing of paraffin blocks

From:"Morken, Tim"

Melanie, For HIV detection we use the DAKO monoclonal antibody HIV(p24)cat#
M857 for all formalin-fixed, paraffin-embedded samples. We use it with
protienase K digestion (0.1mg/ml in 0.2M TRIS buffer pH 7.5) for 15 min at
room temp, primary incubated at 1:10 for one hour. Detection is with DAKO
LSAB2 Alk phos kit and DAKO fast red.

Tim Morken EMT(MSA), HTL(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology
1600 Clifton Road
Atlanta, GA 30333

PH 404-639-3964
FAX 404-639-3043

-----Original Message-----
From: Trivett Melanie []
Sent: Thursday, January 23, 2003 9:13 PM
To: HistoNet Server
Subject: HIV testing of paraffin blocks

Does anyone out there know if is possible to determine HIV status on a
formalin-fixed paraffin embedded sample?  Perhaps by PCR methods? I have
seen a few references but wonder if this is being done in a diagnostic

Any information you can provide would be greatly appreciated.

Many thanks,


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