RE: Cutting artifact with muscle biopsies

From:"Gladney, Diane C Ms MACH"


We have had the same problem, too. I have tried everything and nothing seems
to eliminate the random problem. Hopefully, someone on Histonet will have
conquered this problem or at least been able to better manage it. If so,
please share with all of us who are at our wits end. The bottom line here is
that the pathologists and myself have resigned ourselves to add this problem
to the list of things that are unexplained and so far haven't been able to
correct. It is like some of the things in life that you just have to accept
and live with until technology or better minds than ours can come up with a


Diane C. Gladney, HT (ASCP)
Histology Supervisor
Moncrief Army Community Hospital
P.O. BOX 484
4500 Stuart Ave.
FT. Jackson, SC 29207

(803) 751-2530
DSN 734-2530


-----Original Message-----
From: Hart, Lorraine []
Sent: Friday, January 24, 2003 9:25 AM
To: ''
Subject: Cutting artifact with muscle biopsies

I am looking for a reason and a solution to a problem I am having with
frozen muscle biopsies.  The term we use is pie crust artifact, but
basically it looks like hills and valleys on the tissue.  The tissue is
rippled like it didn't stay put on the slide.  I thought it was static (but
we clean with acetone between each section, and use an anti-static brush in
the cryostat) or perhaps the type of coated slides we use but have tried
AES, Chrome, and commercial charged slides, and no coating at all with the
same effect.  This does not happen with every case or even within frozen
blocks of the same case, it does not matter who cuts the sections, this
artifact sometimes appears.  Within the sections there are some nice flat
areas of muscle and then areas where it is completely rippled, but each
slide is different.  As you can see I am having a real problem with this for
quite some time now, if anyone has any ideas or hints I would welcome them.
Has anyone ever come across this before?  Thanks.

Lorraine Hart, MLT
Senior Technologist in Charge
Pathology Laboratory
(613) 737-7600 X2241

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