RE: Best fixative for LCM

From:"Johnson, Teri"


It depends on what you're looking for.  I have a paper from J Histochem Cytochem 10(9): 1237-1245, 2002 in which they paraffin process Methacarn-fixed tissues for Genomic DNA analysis.  I haven't tried it myself but figured it might be worth a look.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

-----Original Message-----
From: Mark Elliott []
Sent: Friday, January 31, 2003 9:13 AM
Subject: Best fixative for LCM

I have been asked by another researcher here to posat this.  What have
people doing LCM found was the best fixative for capturing cells. 
Currently our material is formalin-fixed and paraffin-embedded (using
archived tissues).  We are going to be recieving some new tissue and
will need to paraffin-embed it, however we are wondering what the best
fixative would be-formalin, Carnoy's, alcohol?- keeping in mind that we
also need good morphology.

Thanks for any and all suggestions.

W. Mark Elliott, PhD
Research Associate
UBC-McDonald  Research Lab
St. Paul's Hospital
Vancouver, BC

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