RE: Best fixative for LCM

From:Luis Chiriboga

the question revolves around whether you want to collect RNA or DNA.....
check out this article: Am J Pathol 2001, 158:419-429
If you cant,  I have it in PDF format and can email direct.

-----Original Message-----
From: Mark Elliott []
Sent: Friday, January 31, 2003 10:13 AM
Subject: Best fixative for LCM

I have been asked by another researcher here to posat this.  What have
people doing LCM found was the best fixative for capturing cells. 
Currently our material is formalin-fixed and paraffin-embedded (using
archived tissues).  We are going to be recieving some new tissue and
will need to paraffin-embed it, however we are wondering what the best
fixative would be-formalin, Carnoy's, alcohol?- keeping in mind that we
also need good morphology.

Thanks for any and all suggestions.

W. Mark Elliott, PhD
Research Associate
UBC-McDonald  Research Lab
St. Paul's Hospital
Vancouver, BC

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