RE: Best fixative for LCM
the question revolves around whether you want to collect RNA or DNA.....
check out this article: Am J Pathol 2001, 158:419-429
If you cant, I have it in PDF format and can email direct.
From: Mark Elliott [mailto:MElliott@mrl.ubc.ca]
Sent: Friday, January 31, 2003 10:13 AM
Subject: Best fixative for LCM
I have been asked by another researcher here to posat this. What have
people doing LCM found was the best fixative for capturing cells.
Currently our material is formalin-fixed and paraffin-embedded (using
archived tissues). We are going to be recieving some new tissue and
will need to paraffin-embed it, however we are wondering what the best
fixative would be-formalin, Carnoy's, alcohol?- keeping in mind that we
also need good morphology.
Thanks for any and all suggestions.
W. Mark Elliott, PhD
UBC-McDonald Research Lab
St. Paul's Hospital
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