RE: Bacteria Stain

Noreen Gilman, BS,HT(ASCP)QIHC
Histopathology Supervisor
Broward General Medical Center
Ft. Lauderdale, FL 33316
954.355.4358 Phone
954.355.4139 Fax
954.387.0213 Pager

>>> Mike & Phyllis Thaxton <thaxfax@msn.com> 01/21/03 08:29PM >>>
Can you send a copy of the procedure. Our B&B isn't the best either.

Phyllis

>From: Cindy/Rick DuBois <ccdub@earthlink.net>
>To: "Dunn-Jena, Patsy A" <padunnje@iupui.edu>, histonet@pathology.swmed.edu
>Subject: Re: Bacteria Stain
>Date: Tue, 21 Jan 2003 10:41:02 -0800 (PST)
>
>We use a McDonalds Gram Stain. It works beautifully and only takes 5
>minutes. It uses:
>Carbol Fuschin
>Gentian violet
>Grams decolorizer
>Grams Iodine
>Tartrazine.
>Our doctors love this over the brown & brenn
>
>-------Original Message-------
>From: "Dunn-Jena, Patsy A" <padunnje@iupui.edu>
>Sent: 01/16/03 12:30 PM
>To: histonet@pathology.swmed.edu
>Subject: Bacteria Stain
>
> >
> > Need to know which stain most of you feel most comfortable with to check
>for bacteria. Was instructed to do a Brown & Brenn which has not come out
>to
>my satisfaction any of the 3 times I have done it. FINALLY have them
>convinced to let me try a Brown & Hopps. Any words of advice would be
>greatly
>appreciated. This is being done on Formalin Fixed, EDTA decalcified
>paraffin embedded bone/tooth sections.
>
>Patsy Dunn-Jena, RVT, LAT, HT (ASCP)
>Indiana University School of Dentistry
>Mineralized Tissue and Histology Research Laboratory
>Indianapolis, IN
>padunnje@iupui.edu