Patsy:

We do the Brown and Brenn almost exclusively.  We are occasionally asked to
do a Brown-Hopps but only in rare instances.  But we almost never have any
bone specimens.

Jeanine Bartlett
CDC, Atlanta

-----Original Message-----
From: Dunn-Jena, Patsy A
To: histonet@pathology.swmed.edu
Sent: 1/16/2003 3:30 PM
Subject: Bacteria Stain

Need to know which stain most of you feel most comfortable with to check
for bacteria.  Was instructed to do a Brown & Brenn which has not come
out to my satisfaction any of the 3 times I have done it.  FINALLY have
them convinced to let me try a Brown & Hopps.  Any words of advice would
be greatly appreciated.  This is being done on Formalin Fixed, EDTA
decalcified paraffin embedded bone/tooth sections. 

Patsy Dunn-Jena, RVT, LAT, HT (ASCP)
Indiana University School of Dentistry
Mineralized Tissue and Histology Research Laboratory
Indianapolis, IN
padunnje@iupui.edu