Leydig cells and LCM

From:Renee Thacker

I am trying to stain Leydig cells for pickup using laser capture microdissection.  I have tryed oil red o which stained the cells beautifully but I can not seem to get this stain to work with LCM since oil red o is removed in alcohol and I can't get tissue to lift from the slide without taking it through dehydrating alcohols.
So the question is:
Could someone recommend a stain that will help differentiate Leydig cells in fetal rat testis from other cells.?
Could does anyone have a protocol for oil red o or any fat stain that will work with LCM? 
Renee Thacker

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