Fwd: staining JB-4 sections
Techniques in Plastic for Light Microscopy
National Society for Histotechnology - Region IV Symposium
June 25, 1983 Ann Arbor, Michigan
Alexandra N. Brady, M.S., MT/HTL (ASCP)
Robert Schoonhoven, HTL (ASCP)
I like the Lee's Methylene Blue -Basic Fuchsin for an H&E
look-a-like.
1) Stock methylene blue
0.5 g methylene blue (CI 52015)
400 mL distilled water
2) Stock basic fuchsin
0.5 g basic fuchsin (CI 42510)
400 mL distilled water
3) Stock phosphate buffer 0.1 M pH 6.8
49
mL 0.2 M sodium phosphate dibasic anhydrous (28.4 g Na2H2PO4 in 1
liter DH2O)
51
mL 0.2 M sodium phosphate monobasic monohydrate (27.8 g
NaH2PO4-H2O in 1 liter DH2O)
100 mL
distilled water
4) 95% ethyl alcohol
5) Working methylene blue-basic fuchsin
12.0
mL stock methylene blue
12.0
mL stock basic fuchsin
21.0
mL stock phosphate buffer
15.0
mL 95% ethyl alcohol
DISCARD AFTER USE
Procedure:
1) Stain in working solution for 10 seconds
2) Rinse in distilled water
3) Dip in 95% alcohol quickly with agitation to remove background
stain
4) Rinse in water
5) Dry and mount
Results: Nuclei - blue
Cytoplasm, cilia, certain cell granules - shades of red and pink
Cartilage and most cell granules - blue to purple
Reference: Sorvall Instruction Manual, Staining Procedures for
Plastic Embedded Tissue, DuPont Company, Instrument Products, Biomedical
Div., July, 1979
Date: Thu, 30 Jan 2003 16:01:38
+0100
X-PH: V4.1@mailhub3
From: =?iso-8859-2?Q?Michaela_Fr=FDzkov=E1?=
<michaela.fryzkova@centrum.cz>
Subject: staining JB-4 sections
To: histonet <histonet@pathology.swmed.edu>
Dear Colleagues,
I need an advice about staining sections embedded in JB-4 Plus. I embed
in
this medium and now I need stain my sections. I wanted to use
regular
haematoxylin-eosin staining, but it doesn#180#t work. Do you know
something
about that? Can you recommend me some other staining?
Thank you, very much.
Michaela Fryzkova
Department of Parasitology
Faculty of Science
Charles University
Prague
Czech Republic
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