Fwd: fresh nerve staining

From:louise renton

Hi all,

I am reposting this as I am not sure that it was sent, I certainly got no 
replies.

I am posting this for a fellow researcher who is looking for a 
technique/reference to stain nerves in unfixed, fresh bone.

Does such a technique exist? The researcher is currently doing methylene 
blue supravital staining, but this seems to produce a lot of background (ie 
the  bone is staining as well as the nerves) and is therefor not ideal.

Any and all suggestions welcome

TIA
Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"

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