Fw: Bootleg Phosphomolybdic time?
questions 1 & 2 for Russ.
3.) Electron microscopy - yes. I suppose I should come clean on what it
is I'm actually using. When I first tried negative staining back in the
early 1970's my first efforts were pretty abysmal. So, I contacted a
lovely young girl in the University's Virology department who agreed to
introduce me to the technique. Being a naive beginner I thought PTA was
tungstophosphoric acid rather than potassium tungstophosphate. After this
training I bought, at the suggestion of the lythe limbed young sybarite,
a small bottle of both the sodium and potassium salts. Since then I have
been using, when called upon, the sodium salt (STA) as I find it to be
the better stain, in my hands. Over the years since I tried other batches
of the sodium salt but none has been quite as good as my original bottle
so I just keep it and only make small amounts of the stain. Although I
use the generic term, phosphotungstic acid, I'm really using sodium
we're all guilty of this lax use of chemical nomenclature. Fortunately,
my techniques book and formulary make it quite clear what salt I actually
use. My humble apologies for this confusion.
> Good Morning Gentlemen!
Dr. Ian Montgomery,
> I followed your recent brief Histonet exchange concerning
acid. I wonder if I might ask some questions, since I am curious!
> 1) What is it that happens to PMA that makes it
> 2) Russ, what neuro procedure did you use it on?
> 3) Dr Montgomery, you mention "Brilliant for negative staining
particles" for phosphotungstic acid, I take it that is for
> 4) I looked in our Sigma Aldrich catalogue. PMA costs about $460 for
grams, not too different for your cost. I was wondering if I could buy
cheap here and sell it in the UK! Won't work with that price!
> Thank you
> Mike Titford
> USA Pathology
> Mobile AL USA
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Tel: 0141 339 8855
Pager: 07625 702883
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